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HBV Drug Resistance and Genotyping

Clinical System Name

Miscellaneous Test

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA, Red, or Gold SST

Preferred Vol: 3.0 mL

Minimum Vol: 1.5 mL

 

Note:

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: -20 CPA freezer, Send Outs rack.

 

Off-site Collection:

Stability

Specimen Type Temperature Time
Plasma/Serum Room Temp 24 h
  Refrigerated 72 h
  Frozen Indefinitely

 

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Molecular Virology Laboratory

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Send Out Instructions

Reference Test Name: HBV Drug Resistance and Genotyping
Reference Test Code: HBVGTD
Instructions: Send Monday through Friday with the UW courier.

 

Synonyms

HBV Drug Resistance

HBV Genotype

HBV POL Gene Sequencing

Description

HBV Drug Resistance and Genotyping is determined by Sanger sequencing. An 850bp region of the HBV DNA polymerase gene is sequenced, then compared to an HBV genotyping and drug resistance database provided by Genofar, a German, non-profit, scientific society. PCR sensitivity is 75 IU/mL. Minor subpopulations of mutant viruses below 20-25% of the total HBV population may not be detected. Drug resistance analysis includes Lamivudine, Adefovir, Entecavir, Tenofovir, and Telbivudine.

Availability

STAT Performed TAT
N T 2 - 7 d

 

Methodology

Method: Sanger Sequencing

Analytical Volume: 0.7 mL Plasma or Serum

Limitations:

Reference Ranges

Interpretive report provided.

CPT Codes

87912