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Direct Thrombin Inhibitor

Clinical System Name

Miscellaneous Test

Synonyms

Argatroban Level

Bivalrudin Level

Lepirudin Level

Thombrin Inhibitor-Direct

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill.

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix large Cerner label and store in the Core 6 (-70) freezer, Send Out rack.

 

Off-site Collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within ONE hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze two aliquots of 0.6 mL plasma in plastic tubes at -70C.

 

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room temp Unacceptable
  Refrigerated Unacceptable
  Frozen Y

 

Availability

STAT Performed TAT
Y* Daily 1 - 8 d

 

Note: *STAT requests require UW Lab Med Resident (LMR) approval. Please contact the Send Outs department at ext. 7-2563 for STAT requests.

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

CPT Codes

80299

Methodology

Method: Clot Based

Analytical Volume: 1.0 mL Citrate Plasma

Limitations:

Reference Range

Reference values accompany patient report.

Send Out Instructions

Reference Test Name: Direct Thrombin Inhibitor
Reference Test Number: DTI
Instructions: Send out Monday through Friday with the UW/HMC courier.