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Direct Thrombin Inhibitor

Clinical System Name

Miscellaneous Test

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.

Processing Instructions: Main Campus

Deliver whole blood to Coag bench. Coag Technologist will process.

 

Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Processing Instructions: Offsite & Regional Clinics

Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location:  -70 C (preferred) or -20 C. 

 

Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.

 

Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room temp Unacceptable
  Refrigerated Unacceptable
  Frozen Y

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

 

Synonyms

Argatroban Level

Bivalrudin Level

Lepirudin Level

Thombrin Inhibitor-Direct

Availability

STAT Performed TAT
Y* Daily 1 - 8 d

 

Note: *STAT requests require UW Lab Med Resident (LMR) approval. Please contact the Send Outs department at ext. 7-2563 for STAT requests.

Methodology

Method: Clot Based

Analytical Volume: 600 uL plasma

Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

Reference Range

Reference values accompany patient report.

CPT Codes

80299

Send Out Instructions

Reference Test Name: Direct Thrombin Inhibitor
Reference Test Number: DTI
Instructions: Send out Monday through Friday with the UW/HMC courier.