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HelpChromosomes - Fibroblasts
Clinical System Name
Constitutional Karyotype
Synonyms
Fibroblast Karyotyping
Karyotype Fibroblast
Description
This test is usually performed when mosaicism is suspected. Skin or tissue may be cultured in order to establish long term single cell cultures to detect abnormalities in chromosome number, large chromosomal duplications and deletions and other large structural rearrangements. If mosaicism is suspected, biopsies from two sites is recommended.
Tissue samples must be cultured before testing. Please also order Fibroblast Culture.
Sample Requirements
Specimen: Skin or other tissue
Container(s): Cell Marker/Cytogenetics Transport Media (RPMI with fetal calf serum & antibiotics), Fibroblast Transport Media, Hank's Balanced Salt Solution or sterile saline
Preferred Vol: 2.0 - 4.0 mm punch biopsy
Minimum Vol: 2.0 mm punch
Note: Obtain Cell Marker/Cytogenetics Transport Media from Histo Lab (ext. 7-2580). Check tube for expiration date. Specify skin biopsy site. Test request must be completed by the ordering provider. Patient history information is required. Keep sample sterile and at room temperature or refrigerated; do not freeze.
- CPA: transport media is stored in Histology fridge #2. Media is pink in 15 mL tubes with blue screw cap. Check expiration date. Back-up supply in Cell Markers Lab.
- SCH Regional Labs: transport media stored in lab fridge.
Samples received after 3 pm will be set up the following day. Samples must be set-up ASAP, plan collection & shipping accordingly. Samples that are fixed in formalin or formaldehyde will not be accepted.
Processing Instructions
Reject due to: n/a - send to lab
Spin: N
Aliquot: N
Temp: RT
Storage location: Process sample without delay. Days: Transport specimen, requistion, and labels to 4th floor (station #181). Eves/Nights: Store in Molecular Genetics box in CPA refrigerator #2 with labels & requisitions. DO NOT FREEZE.
Off-site collection: Obtain Cell Marker/Cytogenetics Transport Media from Histo Lab (ext. 7-2580). Keep sample sterile. Store and transport sample refrigerated - do not freeze.
Stability
| Temperature | Time |
|---|---|
| Room temp | Shipping ok |
| Refrigerated | Overnight ok |
| Frozen | No |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Daily | 6 weeks |
Performing Laboratory
Seattle Children's Hospital
Department
Department: Cytogenetics Laboratory
Phone number: 206-987-3961
Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org
Lab Genetic Counselors: LabGC@seattlechildrens.org
CPT Codes
88233, 88285, 88262, 88291
Methodology
Method: Tissue culture & Routine G-band analysis
Requisition
Clinical Utility
x
Special Instructions
Specify skin or tissue biopsy site. Test request form must be completed by the ordering provider. Patient history information is required. For additional information or consultation call Children's Cytogenetics Lab at (206) 987-3961. After hours call the Clinical Lab at (206) 987-2102 or the on-call pathologist at (206) 987-2131.