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Cell Sorting with EBV PCR

Important Note

This test requires advance notice to Hematopathologist on-service.  It must be collected Mon - Thurs before 10 am.

Clinical System Name

Miscellaneous Test

Sample Requirements

Specimen: Whole Blood

Container: Lavender/EDTA

Preferred Vol: 15.0 mL

Minimum Vol: 15 mL

Processing Instructions

Reject due to: Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Spin: N

Aliquot: N

Temp: RT

Storage Location: Place in the room temperature Send Outs rack, and notify the Send Outs department.

 

Send-outs laboratory:  Please send whole blood at room temperature to Genetics and Genomics Diagnostic Laboratory (GGDL).  Please contact Sherry Steele, Operations Specialist in GGDL; Ph: 513-636-4474; Sherry.Steele@cchmc.org to coordinate sample delivery.

Stability

Specimen Type Temperature Time
  Room temp 48 h
  Refrigerated Unacceptable
  Frozen Unacceptable

Performing Laboratory

Cincinnati Children's Hospital

GGDL - Sherry Steele

Genetics and Genomics Diagnostic Laboratory (GGDL).

Phone Number: 513-636-4474

Department

Department: Send Outs

Phone Number: (206) 987-2563

Special Instructions

Ordering provider must fill out patient information and diagnosis for the following TWO requisitions: 

 

1) Oncology Genetic Testing Requisition (Oncology Genetic Testing Requisition (testmenu.com)), select “Cell Separation (for non-engraftment testing)” on page 2 of requisition; and

 

2) Molecular and Genomic Pathology services-Infectious Diseases (https://www.cincinnatichildrens.org/service/p/pathology/molecular-pathology/infectious-disease), select “Epstein-Barr Virus (EBV) quantitative.”

 

3)  Please indicate within the Misc order which populations should be sorted: Options for sorting include CD19+ B cells, CD3+ T cells, CD56+ NK cells, CD15+ myeloid and CD138+ plasma cells.  Must also include sorting priority for populations (in case of limited sample volume).

Send Out Instructions

Reference Test Name Cell Separation and EBV Quantitative PCR
Reference Test Code  
Instructions Ship ambient, Monday through Thursday, via FedEx Priority Overnight. Saturday delivery is not acceptable.