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HelpTest Code APCR Activated Protein C Resistance
Clinical System Name
Activated Protein C Resistance
Synonyms
Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance
Factor 5 Leiden Protein Assay
Factor V Leiden Protein Assay
Protein C Cofactor
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within one hour of collection.
Processing Instructions
Main Campus CPA: Core Technologist will process specimen. Deliver blood directly to Core Coag bench.
Reject due to: Clotted, Insufficient quantity (underfill), or Improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Core Technologist: Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Off-site collection: Ship completely frozen on dry ice.
Children's Regional Clinics: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated Platelet-Poor Plasma | Room Temp | N |
| Refrigerated | N | |
| Frozen | Y |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M, Th | 1 - 5 d |
Performing Laboratory
Harborview Medical Center (HMC)
Clinical Coagulation Lab
325 9th Ave
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85307
Methodology
Method: Clot Based
Analytical Volume: 1.0 mL Citrate Plasma
Limitations:
Reference Range
| Female | Male | ||
| Age | Range | Age | Range |
| 0- | Negative | 0- | Negative |
Send Out Instructions
| Reference Test Name: | Activated Protein C Resistance |
| Reference Test Number: | APCR |
| Instructions: | Send out Monday through Friday with the UW courier. |