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Test Code APCR Activated Protein C Resistance

Important Note

This assay can be used during heparin or oral anticoagulant therapy, and is not sensitive to lupus inhibitors.

Clinical System Name

Activated Protein C Resistance

Synonyms

Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance

Factor 5 Leiden Protein Assay

Factor V Leiden Protein Assay

Protein C Cofactor

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, and improper collection.

Spin: N

Aliquot: N

Temp: -70

Storage Location: CPA -70 freezer, Send Out rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within  1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots  0.6 mL, affix large label and store in -70 freezer, Send Out rack. Store remaining labels and copy of requisition in the clear plastic box on the CPA fridge.

 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room Temp Unacceptable
  Refrigerated Unacceptable
  Frozen Y

 

Availability

STAT Performed TAT
N M, Th 1 - 5 d

Performing Laboratory

Harborview Medical Center (HMC)

Clinical Coagulation Lab

325 9th Ave

Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

85307

Methodology

Method: Clot Based

Analytical Volume: 1.0 mL Citrate Plasma

Limitations:

Reference Range

Female Male
Age Range Age Range
0- Negative 0- Negative

 

Send Out Instructions

Reference Test Name: Activated Protein C Resistance
Reference Test Number: APCR
Instructions: Send out Monday through Friday with the UW courier.