Test Code APCR Activated Protein C Resistance
Clinical System Name
Activated Protein C Resistance
Synonyms
Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance
Factor 5 Leiden Protein Assay
Factor V Leiden Protein Assay
Protein C Cofactor
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (3) 1.8 mL or (1) 2.7 mL
Minimum Vol: (2) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within 1 hour of collection.
Processing Instructions
Reject due to: Clotted, hemolyzed, insufficient quantity, and improper collection.
Spin: N
Aliquot: N
Temp: -70
Storage Location: CPA -70 freezer, Send Out rack.
Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots 0.6 mL, affix large label and store in -70 freezer, Send Out rack. Store remaining labels and copy of requisition in the clear plastic box on the CPA fridge.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.
Ship frozen on dry ice.
Stability
Specimen Type | Temperature | Time |
---|---|---|
Citrated Platelet-Poor Plasma | Room Temp | Unacceptable |
Refrigerated | Unacceptable | |
Frozen | Y |
Availability
STAT | Performed | TAT |
---|---|---|
N | M, Th | 1 - 5 d |
Performing Laboratory
Harborview Medical Center (HMC)
Clinical Coagulation Lab
325 9th Ave
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85307
Methodology
Method: Clot Based
Analytical Volume: 1.0 mL Citrate Plasma
Limitations:
Reference Range
Female | Male | ||
Age | Range | Age | Range |
0- | Negative | 0- | Negative |
Send Out Instructions
Reference Test Name: | Activated Protein C Resistance |
Reference Test Number: | APCR |
Instructions: | Send out Monday through Friday with the UW courier. |