Sign in →

Test Code APCR Activated Protein C Resistance

Important Note

This assay cannot be used during heparin or oral anticoagulant therapy, and is sensitive to lupus inhibitors.

Clinical System Name

Activated Protein C Resistance

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within one hour of collection.

Processing Instructions

Main Campus CPA: Core Technologist will process specimen. Deliver blood directly to Core Coag bench.

 

Reject due to: Clotted, Insufficient quantity (underfill), or Improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location: Core 14 Freezer ( -70 C).

 

Core Technologist: Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Off-site collection: Ship completely frozen on dry ice.

 

Children's Regional Clinics: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Citrated Platelet-Poor Plasma Room Temp N
  Refrigerated N
  Frozen Y

 

Performing Laboratory

Harborview Medical Center (HMC)

Clinical Coagulation Lab

325 9th Ave

Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Send Out Instructions

Reference Test Name: Activated Protein C Resistance
Reference Test Number: APCR
Instructions: Send out Monday through Friday with the UW courier.

 

Synonyms

Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance

Factor 5 Leiden Protein Assay

Factor V Leiden Protein Assay

Protein C Cofactor

Availability

STAT Performed TAT
N M, Th 1 - 5 d

Methodology

Method: Clot Based

Analytical Volume: 1.0 mL Citrate Plasma

Limitations:

Reference Range

Female Male
Age Range Age Range
0- Negative 0- Negative

 

CPT Codes

85307