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HelpTest Code APCR Activated Protein C Resistance
Clinical System Name
Activated Protein C Resistance
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated Platelet-Poor Plasma | Room Temp | N |
| Refrigerated | N | |
| Frozen | Y |
Performing Laboratory
Harborview Medical Center (HMC)
Clinical Coagulation Lab
325 9th Ave
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
Synonyms
Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance
Factor 5 Leiden Protein Assay
Factor V Leiden Protein Assay
Protein C Cofactor
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M, Th | 1 - 5 d |
Methodology
Method: Clot Based
Analytical Volume: 600 uL plasma
Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
| Female | Male | ||
| Age | Range | Age | Range |
| 0- | Negative | 0- | Negative |
CPT Codes
85307
Send Out Instructions
| Reference Test Name: | Activated Protein C Resistance |
| Reference Test Number: | APCR |
| Instructions: | Send out Monday through Friday with the UW courier. |