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HelpTest Code BARTONELLA Bartonella Antibody Panel, IgG and IgM, Serum
Reporting Name
Bartonella Ab Panel, IgG and IgMUseful For
Diagnosis of Bartonella infection, especially in the context of a cat scratch
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
0.15 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Day(s) Performed
Monday through Saturday
TAT: 3 - 5 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Immunofluorescence Assay (IFA)
Method Description
The Euroimmun indirect immunofluorescence test is a standardized, in vitro assay for the determination of specific antibodies against Bartonella henselae and Bartonella quintana. BIOCHIP Mosaics are coated with B henselae and B quintana infected cells positioned next to each other in one reaction field. Samples are diluted and incubated on the substrate slides. If the reaction is positive, specific antibodies of class IgG and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and made visible using fluorescence microscopy. Semiquantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Unpublished Mayo method)
Reference Values
BARTONELLA HENSELAE
IgG: <1:128
IgM: <1:20
BARTONELLA QUINTANA
IgG: <1:128
IgM: <1:20
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86611 x 4
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BART | Bartonella Ab Panel, IgG and IgM | 90251-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 15659 | Bart Henselae IgG | In Process |
| 15660 | Bart Henselae IgM | In Process |
| 15661 | Bart Quintana IgG | In Process |
| 15662 | Bart Quintana IgM | In Process |
Report Available
Same day/1 to 3 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Testing Algorithm
For information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.
Special Instructions
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.