Sign in →

Test Code BARTONELLA Bartonella Antibody Panel, IgG and IgM, Serum

Reporting Name

Bartonella Ab Panel, IgG and IgM

Useful For

Diagnosis of Bartonella infection, especially in the context of a cat scratch


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Type

Serum

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Day(s) and Time(s) Performed

Monday through Saturday; 9 a.m.

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Immunofluorescence Assay (IFA)

Method Description

The Euroimmun indirect immunofluorescence test is a standardized in virto assay for the determination of specific antibodies against Bartonella henselae and B quintana. BIOCHIP Mosaics are coated with B henselae and B quintana infected cells positioned next to each other in one reaction field. Samples are diluted and incubated on the substrate slides. If the reaction is positive, specific antibodies of class IgG and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and made visible using fluorescence microscopy. Semi quantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(General instructions for Euroimmun indirect immunofluorescence tests version 1/31/2017; IIFT Bartonella henselae/Bartonella quintana (IgM) Instructions for the indirect immunofluorescence test. Version 7/18/2017).

Reference Values

Bartonella henselae

IgG: <1:128

IgM: <1:20

 

Bartonella quintana

IgG: <1:128

IgM: <1:20

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86611 x 4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BART Bartonella Ab Panel, IgG and IgM 90251-0

 

Result ID Test Result Name Result LOINC Value
15659 Bart Henselae IgG 6954-2
15660 Bart Henselae IgM 6955-9
15661 Bart Quintana IgG 44827-4
15662 Bart Quintana IgM 44825-8

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.