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Test Code C2 Func C2 Complement, Functional, Serum

Reporting Name

C2 Complement, Functional, S, NR
Seattle Children's Hospital Note:

Clinical System Name: C2 Complement, Functional

Useful For

Investigation of a patient with a low (absent) hemolytic complement (CH50)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum Red


Advisory Information


The total complement assay (COM / Complement, Total, Serum) should be used as a screen for suspected complement deficiencies before ordering individual complement component assays. A deficiency of an individual component of the complement cascade will result in an undetectable total complement level.

To evaluate for C2, C3, and C4 in one orderable, consider ordering C2 / C2 Complement, Functional, with Reflex, Serum.



Specimen Required


Patient Preparation: Fasting preferred but not required

Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Red top

Submission Container/Tube: Plastic, 5-mL tube (T465)

Specimen Volume: 1 mL

Collection Instructions:

1. Immediately after drawing the specimen, place the tube on wet ice.

2. Spin down and separate serum from clot.

3. Immediately freeze specimen.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen 21 days

Reference Values

25-47 U/mL

Day(s) and Time(s) Performed

Monday through Saturday; 3 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86161

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C2FXN C2 Complement, Functional, S, NR In Process

 

Result ID Test Result Name Result LOINC Value
C2FX C2 Complement,Functional,S No LOINC Needed
INT53 Interpretation 69048-7

Method Description

C2 complement activity is measured by mixing patient serum with a C2-deficient serum. The lytic activity of the serum mixture is tested against sensitized, labeled liposomes. If lysis occurs, the patient serum must be the source of the C2. The target liposomes are a commercial reagent (WAKO total complement CH50), and the assay is performed on a Siemens Advia 1200.(Unpublished Mayo information)

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross OK

Other

Serum gel tube

Method Name

Automated Liposome Lysis Assay