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Test Code Chrom F8 Factor 8 Assay, Chromogenic

Clinical System Name

Factor 8 Assay, Chromogenic

Synonyms

Chromogenic Factor 8
Factor 8, Chromogenic
Factor VIII Chromogenic
Refacto Assay (Recombinant F8)

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within one hour of collection.

Processing Instructions

Deliver blood to Core Coag bench. Coag Technologist will process.

 

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  Drawn daily; Performed  M & Th at HMC Coag Lab 5 - 10 d

 

Performing Laboratory

University of Washington

Dept. of Laboratory Medicine

Harborview Medical Center Coag Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department:  Send Outs

 

Phone Number: (206) 987-2563

 

CPT Codes

85130

 

Methodology

Method: Optical

Analytical Volume: 1.0 mL citrate plasma

Limitations:

Reference Range

50 - 234 %

 

Send Out Instructions

 

Reference Test Name: Chromogenic Factor 8
Reference Test Number: CHRF8
Instructions: Send out M - F with the UW courier.