Test Code Chrom F8 Factor 8 Assay, Chromogenic
Clinical System Name
Factor 8 Assay, Chromogenic
Synonyms
Chromogenic Factor 8
Factor 8, Chromogenic
Factor VIII Chromogenic
Refacto Assay (Recombinant F8)
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (3) 1.8 mL or (1) 2.7 mL
Minimum Vol: (2) 1.8 mL
Note:
Processing Instructions
Reject due to: Clotted, Hemolyzed, Insufficient quantity, and Improper collection.
Spin: N
Aliquot: N
Temp: RT
Storage location: Core 6 Freezer ( -70 C) Send Out rack.
Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots 0.6 mL, affix large label and store in -70 freezer, Send Out rack.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.
Ship frozen on dry ice.
Stability
Specimen Type | Temperature | Time |
---|---|---|
Citrated platelet-poor plasma | Room temp | N |
Refrigerated | N | |
Frozen | Y |
Availability
STAT | Performed | TAT |
---|---|---|
N | Drawn daily; Performed M & Th at HMC Coag Lab | 5 - 10 d |
Performing Laboratory
University of Washington
Dept. of Laboratory Medicine
Harborview Medical Center Coag Lab
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85130
Methodology
Method: Optical
Analytical Volume: 1.0 mL citrate plasma
Limitations:
Reference Range
50 - 234 % |
Send Out Instructions
Reference Test Name: | Chromogenic Factor 8 |
Reference Test Number: | CHRF8 |
Instructions: | Send out M - F with the UW courier. |