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Test Code D-Lactate D-Lactate, Plasma

Important Note

This test is NOT available for collection at Seattle Childrens. It requires collection in a grey-top tube that is not stocked at Seattle Children's locations. Urine d-lactate is the preferred test for d-lactate acidosis.

Reporting Name

D-Lactate, P

Useful For

An adjunct to urine D-lactate (preferred) for the diagnosis of D-lactate acidosis


Ordering Guidance


Urine is the preferred specimen for D-lactate determination, order DLAU / D-Lactate, Urine.

 

For determination of L-lactate (lactic acid), order LACS1 / Lactate, Plasma



Specimen Required


Collection Container/Tube: Sodium Fluoride/Potassium Oxalate Tube, 2 mL (T275)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge, aliquot plasma into plastic vial, and freeze immediately.


Seattle Children's Hospital Note:

Obtain Grey Top from Main Lab. Collect 2.0 mL whole blood in a Grey top.

Specimen Type

Plasma NaFl-KOx

Specimen Minimum Volume

0.55 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma NaFl-KOx Frozen (preferred) 365 days
  Ambient  7 days
  Refrigerated  7 days

Day(s) Performed

Wednesday, Friday

Seattle Children's Hospital Note:

TAT: 6 - 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Enzymatic

Method Description

D-lactate is oxidized to pyruvate in the presence of D-lactate dehydrogenase and nicotinamide adenine dinucleotide (NAD+). The reaction proceeds because the pyruvate is continually removed as a pyruvate-hydrazone complex. The quantity of NADH produced is directly proportional to the amount of D-lactate oxidized and is measured spectrophotometrically at 340 nm.(Brandt RB, Siegel SA, Waters MG, Bloch MH: Spectrophotometric assay for D-(-)-lactate in plasma. Anal Biochem.1980;102(1):39-46; Cowan T, Pasquali M: Laboratory investigations of inborn errors of metabolism. In: K Sarafoglou, GF Hoffman, KS Roth, eds. Pediatric Endocrinology and Inborn Errors of Metabolism. 2nd ed. McGraw-Hill Education; 2017:1139-1158)

Reference Values

0.0-0.25 mmol/L

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83605

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLAC D-Lactate, P 14045-9

 

Result ID Test Result Name Result LOINC Value
8878 D-Lactate, P 14045-9

Report Available

4 to 8 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK