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Test Code D-Lactate D-Lactate, Plasma

Important Note

This test is NOT available for collection at Seattle Childrens. It requires collection in a grey-top tube that is not stocked at Seattle Children's locations. Urine d-lactate is the preferred test for d-lactate acidosis.

Reporting Name

D-Lactate, P

Useful For

An adjunct to urine D-lactate (preferred), in the diagnosis of D-lactate acidosis

Necessary Information

For L-lactate (lactic acid), order LAA / Lactate, Plasma

Specimen Required

Collection Container/Tube: Grey top (potassium oxalate/sodium fluoride) (T275)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Spin down and immediately freeze specimen.

Additional Information: For L-lactate (lactic acid), order LLA / Lactate, Plasma.

Seattle Children's Hospital Note:

Obtain Grey Top from Main Lab. Collect 2.0 mL whole blood in a Grey top.

Specimen Type

Plasma NaFl-KOx

Specimen Minimum Volume

0.55 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma NaFl-KOx Frozen (preferred) 365 days
  Ambient  7 days
  Refrigerated  7 days

Day(s) and Time(s) Performed


Seattle Children's Hospital Note:

TAT: 6 - 10 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name


Method Description

D-lactate is oxidized to pyruvate in the presence of D-lactate dehydrogenase and nicotinamide adenine dinucleotide phosphate (NAD). The reaction proceeds because the pyruvate is continually removed as a pyruvate-hydrazone complex. The quantity of reduced NAD produced is directly proportional to the amount of D-lactate oxidized and is measured spectrophotometrically at 340 nm.(Oh MS, Phelps KR, Traube M, et al: D-Lactic acidosis in a man with the short-bowel syndrome. N Engl J Med 1979;301:249-252; Dahlquist NR, Perrault J, Callaway CW, Jones JD: D-Lactic acidosis and encephalopathy after jejunoileostomy: response to overfeeding and to fasting in humans. Mayo Clin Proc 1984;59:141-145)

Reference Values

0.0-0.25 mmol/L

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLAC D-Lactate, P 14045-9


Result ID Test Result Name Result LOINC Value
8878 D-Lactate, P 14045-9

Reject Due To


Mild OK; Gross OK


Mild OK; Gross OK


Mild OK; Gross OK



Testing Algorithm

DLAU / D-Lactate, Urine is the preferred specimen for D-lactate determinations.