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HelpTest Code DIAZ G Diazepam and Metabolites, Serum
Reporting Name
Diazepam and metabolites, SUseful For
Assessing compliance
Monitoring for appropriate therapeutic level
Assessing diazepam toxicity
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Type
Serum RedSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Day(s) Performed
Wednesday
TAT: 3 - 9 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
The internal standard mixture containing chlordiazepoxide-d5, diazepam-d5 , nordiazepam-d5, oxazepam-d5, and temazepam-d5 is added to serum samples, mixed, and centrifuged. The supernatant is diluted and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)
Reference Values
Therapeutic concentrations
Diazepam: Not established
Diazepam and Nordiazepam: 100-2,500 ng/mL
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Diazepam: 10 ng/mL
Nordiazepam: 10 ng/mL
Oxazepam: 10 ng/mL
Temazepam: 10 ng/mL
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80299
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DIA | Diazepam and metabolites, S | 49044-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8629 | Diazepam | 3548-5 |
| 2475 | Nordiazepam | 3537-8 |
| 2459 | Diazepam and Nordiazepam | 16757-7 |
| 622868 | Oxazepam | 3886-9 |
| 622869 | Temazepam | 10343-2 |
Report Available
3 to 7 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.