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HelpTest Code DIAZ G Diazepam and Nordiazepam, Serum
Test Down Notes
This test is temporarily unavailable due to instrumentation issues. As an alternate, order ZW86 (1501SP). For additional details, see test update here.
Reporting Name
Diazepam and Nordiazepam, SUseful For
Assessing compliance
Monitoring for appropriate therapeutic level
Assessing diazepam toxicity
Specimen Required
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Type
Serum RedSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 14 days | |
| Ambient | 14 days | ||
| Frozen | 14 days |
Day(s) Performed
Monday, Wednesday
TAT: 3 - 9 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
The internal standard mixture containing chlordiazepoxide-d5, diazepam-d4, and nordiazepam-d5 is added to serum samples. The serum samples are treated with phosphate buffer and extracted via liquid/liquid extraction. The organic layer from the extraction is dried under nitrogen, reconstituted, and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)
Reference Values
Therapeutic concentrations
Diazepam and Nordiazepam: 200-2,500 ng/mL
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80299
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DIA | Diazepam and Nordiazepam, S | 49044-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8629 | Diazepam | 3548-5 |
| 2475 | Nordiazepam | 3537-8 |
| 2459 | Diazepam and Nordiazepam | 16757-7 |
Report Available
3 to 7 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Neurology Specialty Testing Client Test Request (T732)
-Therapeutics Test Request (T831)