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D-Lactate, Urine

Reporting Name

D-Lactate, U

Useful For

Preferred test for diagnosing D-lactate acidosis, especially in patients with jejunoileal bypass and short-bowel syndrome


Specimen Required


Container/Tube: Plastic, 10-mL urine tube (T068)

Specimen Volume: 2.5 mL

Collection Instructions:

1. Collect a timed or random urine specimen.

2. No preservative.

3. Immediately freeze specimen.


Specimen Type

Urine

Specimen Minimum Volume

0.65 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Frozen (preferred) 365 days
  Refrigerated  7 days
  Ambient  72 hours

Day(s) Performed

Wednesday, Friday

Seattle Children's Hospital Note:

TAT: 6 - 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Enzymatic

Method Description

D-lactate is oxidized to pyruvate in the presence of D-lactate dehydrogenase and nicotinamide adenine dinucleotide (NAD+). The reaction proceeds because the pyruvate is continually removed as a pyruvate-hydrazone complex. The quantity of NADH produced is directly proportional to the amount of D-lactate oxidized and is measured spectrophotometrically at 340 nm.(Unpublished Mayo method based on Brandt approach[1])

Reference Values

0.0-0.25 mmol/L

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83605

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLAU D-Lactate, U 14046-7

 

Result ID Test Result Name Result LOINC Value
8873 D-Lactate, U 14046-7

Report Available

4 to 8 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.