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HelpD-Lactate, Urine
Reporting Name
D-Lactate, UUseful For
Preferred test for diagnosing D-lactate acidosis, especially in patients with jejunoileal bypass and short-bowel syndrome
Specimen Required
Supplies: Urine Tubes, 10 mL (T068)
Container/Tube: Plastic urine tube
Specimen Volume: 0.50 mL
Collection Instructions:
1. Collect a timed or random urine specimen.
2. No preservative.
3. Immediately freeze specimen.
Specimen Type
UrineSpecimen Minimum Volume
0.15 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Frozen (preferred) | 90 days |
| Ambient | 90 days | |
| Refrigerated | 90 days |
Day(s) Performed
Monday, Thursday
TAT: 6 - 10 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Gas Chromatography Mass Spectrometry (GCMS) Stable Isotope Dilution Analysis
Method Description
Urine is spiked with a mixture of internal standards and evaporated. The dry residue is derivatized with DATAN to form the diastereomeric molecules, then acidified and extracted with ethyl acetate. After evaporation, the dry residue is again derivatized to form trimethylsilyl (TMS) esters at the carboxylic acid moiety. Specimens are then analyzed by capillary gas chromatography mass spectrometry selected ion monitoring using positive chemical ionization with chromatographic separation of the L,L- and D,L-diastereoisomers of derivatized lactate, corresponding to L-Lactate and D-Lactate, respectively.(Ding X, Lin S, Weng H, Liang J. Separation and determination of the enantiomers of lactic acid and 2-hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry. J Sep Sci. 2018;41(12):2576-2584)
Reference Values
0.0-0.25 mmol/L
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83605
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DLAU | D-Lactate, U | 14046-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8873 | D-Lactate, U | 14046-7 |