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D-Lactate, Urine

Reporting Name

D-Lactate, U

Useful For

Preferred test for diagnosing D-lactate acidosis, especially in patients with jejunoileal bypass and short-bowel syndrome


Specimen Required


Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic urine tube

Specimen Volume: 0.50 mL

Collection Instructions:

1. Collect a timed or random urine specimen.

2. No preservative.

3. Immediately freeze specimen.


Specimen Type

Urine

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Frozen (preferred) 90 days
  Ambient  90 days
  Refrigerated  90 days

Day(s) Performed

Monday, Thursday

Seattle Children's Hospital Note:

TAT: 6 - 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Gas Chromatography Mass Spectrometry (GCMS) Stable Isotope Dilution Analysis

Method Description

Urine is spiked with a mixture of internal standards and evaporated. The dry residue is derivatized with DATAN to form the diastereomeric molecules, then acidified and extracted with ethyl acetate. After evaporation, the dry residue is again derivatized to form trimethylsilyl (TMS) esters at the carboxylic acid moiety. Specimens are then analyzed by capillary gas chromatography mass spectrometry selected ion monitoring using positive chemical ionization with chromatographic separation of the L,L- and D,L-diastereoisomers of derivatized lactate, corresponding to L-Lactate and D-Lactate, respectively.(Ding X, Lin S, Weng H, Liang J. Separation and determination of the enantiomers of lactic acid and 2-hydroxyglutaric acid by chiral derivatization combined with gas chromatography and mass spectrometry. J Sep Sci. 2018;41(12):2576-2584)

Reference Values

0.0-0.25 mmol/L

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83605

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLAU D-Lactate, U 14046-7

 

Result ID Test Result Name Result LOINC Value
8873 D-Lactate, U 14046-7

Report Available

3 to 6 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.