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Test Code F8 INH Factor 8 Inhibitor Level

Important Note

Order this test if the patient has had a previously positive Factor 8 Inhibitor Screen and/or Titer. Available stat with pre-approval.

Clinical System Name

Factor 8 Inhibitor Level

Synonyms

Anti Factor 8 - Quant
F8 Inhib Level
Factor 8 Inhibitor Titer

Factor 8 inhibitor assay

Factor VIII Bethesda

Factor VIII inhibitor titer

Factor VIII inhibitor level

 

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be processed within one hour of collection. 

Processing Instructions

Deliver blood to Core Coag bench. Coag Technologist will process.

 

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
Y w/LMR approval  M - F 5 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

 

CPT Codes

85335

Methodology

Method: Bethesda Assay using

Analytical Volume: 1.0 mL Plasma

Limitations:

Reference Range

None detected.

Note: Values reported in Bethesda units.

Send Out Instructions

Reference Test Name: Factor VIII Inhibitor
Reference Test Number: F8INH
Instructions: See protocol for STAT sendouts. If not ordered STAT, send out Monday through Friday with the UW courier.