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Test Code F8 INH Factor 8 Inhibitor Level

Important Note

Order this test if the patient has had a previously positive Factor 8 Inhibitor Screen and/or Titer. Available stat with pre-approval.

Clinical System Name

Factor 8 Inhibitor Level

Synonyms

Anti Factor 8 - Quant
F8 Inhib Level
Factor 8 Inhibitor Titer

Factor 8 inhibitor assay

Factor VIII Bethesda

Factor VIII inhibitor titer

Factor VIII inhibitor level

 

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the  collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage location: CPA -70 freezer, Send Outs rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix with a large Cerner label and store in -70 freezer, Send Outs rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within ONE of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large Cerner label. Freeze two aliquots of 0.6 mL plasma in aliquot tubes at -70C. Ship frozen on dry ice.

 

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
Y w/LMR approval  M - F 5 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

 

Reference Range

None detected.

Note: Values reported in Bethesda units.

Methodology

Method: Bethesda Assay using

Analytical Volume: 1.0 mL Plasma

Limitations:

CPT Codes

85335

Send Out Instructions

Reference Test Name: Factor VIII Inhibitor
Reference Test Number: F8INH
Instructions: See protocol for STAT sendouts. If not ordered STAT, send out Monday through Friday with the UW courier.