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HelpTest Code F8 INH Factor 8 Inhibitor Level
Clinical System Name
Factor 8 Inhibitor Level
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Required Vol: (1) 2.7 mL
Minimum Vol: N
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Required plasma volume: 1.0 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | N |
| Refrigerated | N | |
| Frozen | Y |
Performing Laboratory
Harborview Medical Center
325 9th Ave, Room GWH47
Seattle, WA 98104
Phone Number: (206) 744-3451
Department
Department: Send Outs
Phone Number: (206) 987-2563
Synonyms
Anti Factor 8 - Quant
F8 Inhib Level
Factor 8 Inhibitor Titer
Factor 8 inhibitor assay
Factor VIII Bethesda
Factor VIII inhibitor titer
Factor VIII inhibitor level
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y w/LMR approval | M - F | 5 d |
Methodology
Method: Bethesda Assay
Analytical Volume: 1.0 mL Plasma
Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
None detected.
Note: Values reported in Bethesda units.
CPT Codes
85335
Send Out Instructions
| Reference Test Name: | Factor VIII Inhibitor |
| Reference Test Number: | F8INH |
| Instructions: | See protocol for STAT sendouts. If not ordered STAT, send out Monday through Friday with the UW courier. |