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Test Code FACTOR 2 Factor 2 Assay

Important Note

Method is affected by hemolysis and improper specimen handling. Patients on Direct Thrombin Inhibitors (Novastan, argatroban, bivalirudin) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Factor 2 Assay

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions. 

Processing Instructions: Main Campus

Deliver whole blood to Coag bench. Coag Technologist will process.

 

Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Processing Instructions: Offsite & Regional Clinics

Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location:  -70 C (preferred) or -20 C. 

 

Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.

 

Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

 

 

Send Out Instructions

Reference Test Name: Factor II Activity
Reference Test Number: F2
Instructions: Ship frozen on dry ice. Send out Monday through Friday with the UW courier.

 

Synonyms

Factor 2 Activity
Factor II Activity

Availability

STAT Performed TAT
N M - F 1 - 3 d

 

Methodology

Method:  Electromagnetic mechanical clot detection assay by STA-R MAX

Analytical Volume: 500 uL plasma

Limitations: Method is affected by hemolysis, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.

Reference Range

Age % Activity
0 - 1 month 33 - 115
1 - 3 months 34 - 115
3 - 6 months 45 - 115
6 months - Adult 50 - 115

 

CPT Codes

85210