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Test Code FACTOR 2 Factor 2 Assay

Important Note

Patients on hirudin (Refludan) and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Factor 2 Assay

Synonyms

Factor 2 Activity
Factor II Activity

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size two French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill.

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: N

Aliquot: N

Temp: -70 C

Storage location: Core 6 Freezer  (-70 C) Send Outs rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix large Cerner label, and store in -70 freezer, Send Outs rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size two French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within ONE hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze two aliquots of 0.6 mL plasma in plastic tubes at -70 C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

 

 

Reference Range

Age % Activity
0 - 1 month 33 - 115
1 - 3 months 34 - 115
3 - 6 months 45 - 115
6 months - Adult 50 - 115

 

Methodology

Method: Stago STA Clottable Factor Assay

Analytical Volume: None Specified

Limitations:

CPT Codes

85210

Send Out Instructions

Reference Test Name: Factor II Activity
Reference Test Number: F2
Instructions: Ship frozen on dry ice. Send out Monday through Friday with the UW courier.