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Test Code FACTOR 5 Factor 5 Activity

Important Note

This test is often confused with a Factor V Leiden (DNA) Test. Method is affected by hemolysis and improper specimen handling. Patients on Direct Thrombin Inhibitors (Novastan, argatroban, bivalirudin) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Coagulation Factor 5 Activity

Synonyms

Coagulation Factor 5 Activity
Coagulation Factor V Activity

Factor 5

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within one hour of collection

Processing Instructions

Main Campus CPA: Core Technologist will process specimen. Deliver blood directly to Core Coag bench.

 

Reject due to: Clotted, Insufficient quantity (underfill), or Improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location: Core 14 Freezer ( -70 C).

 

Core Technologist: Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Off-site collection: Ship completely frozen on dry ice.

 

Children's Regional Clinics: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

85220

Methodology

Method: Stago STA Clottable Factor assay

Analytical Volume:

Limitations:

Reference Range

Units: %
Female Male
Age Range Age Range
0- 50 - 150 0- 50 - 150

 

Send Out Instructions

Reference Test Name: Factor V Activity
Reference Test Number: F5
Instructions: Ship frozen on dry ice. Send out Monday through Friday with the UW courier.