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Test Code FACTOR 5 Factor 5 Activity

Important Note

This test is often confused with a Factor V Leiden (DNA) Test. Method is affected by improper collection, processing or storage, and hemolysis. Patients on hirudin (Refludan) and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Coagulation Factor 5 Activity

Synonyms

Coagulation Factor 5 Activity
Coagulation Factor V Activity

Factor 5

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the  collection of coag testing is not recommended.

 

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage location: CPA -70 freezer, Send Outs rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix with a large Cerner label and store in -70 freezer, Send Outs rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within ONE of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large Cerner label. Freeze two aliquots of 0.6 mL plasma in aliquot tubes at -70C. Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

Reference Range

Units: %
Female Male
Age Range Age Range
0- 50 - 150 0- 50 - 150

 

Methodology

Method: Stago STA Clottable Factor assay

Analytical Volume:

Limitations:

CPT Codes

85220

Send Out Instructions

Reference Test Name: Factor V Activity
Reference Test Number: F5
Instructions: Ship frozen on dry ice. Send out Monday through Friday with the UW courier.