Test Code FACTOR 5 Factor 5 Activity
Clinical System Name
Coagulation Factor 5 Activity
Synonyms
Coagulation Factor 5 Activity
Coagulation Factor V Activity
Factor 5
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (3) 1.8 mL or (1) 2.7 mL
Minimum Vol: (2) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within ONE hour of collection.
Processing Instructions
Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.
Spin: Y
Aliquot: Y
Temp: -70 C
Storage location: CPA -70 freezer, Send Outs rack.
Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix with a large Cerner label and store in -70 freezer, Send Outs rack.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within ONE of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large Cerner label. Freeze two aliquots of 0.6 mL plasma in aliquot tubes at -70C. Ship frozen on dry ice.
Stability
Specimen Type | Temperature | Time |
---|---|---|
Citrated platelet-poor plasma | Room temp | N |
Refrigerated | N | |
Frozen | Y |
Availability
STAT | Performed | TAT |
---|---|---|
N | M - F | 1 - 3 d |
Performing Laboratory
Harborview Medical Center
325 9th Ave, Room GWH47
Seattle, WA 98104
Phone Number: (206) 744-3451
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85220
Methodology
Method: Stago STA Clottable Factor assay
Analytical Volume:
Limitations:
Reference Range
Units: % | |||
Female | Male | ||
Age | Range | Age | Range |
0- | 50 - 150 | 0- | 50 - 150 |
Send Out Instructions
Reference Test Name: | Factor V Activity |
Reference Test Number: | F5 |
Instructions: | Ship frozen on dry ice. Send out Monday through Friday with the UW courier. |