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Test Code FACTOR 9 Factor 9 Assay

Important Note

Method is affected by hemolysis and improper specimen handling. Patients on hirudin (Refludan) and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations

Clinical System Name

Factor 9 Assay

Synonyms

Factor 9 Activity
Factor IX Activity
Factor IX Assay

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within ONE hour of collection.

Processing Instructions

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: Core 6 Freezer ( -70 C) Send Out rack.

 

Note:  Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within  1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots  0.6 mL, affix large label and store in -70 freezer, Send Out rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

 

 

Department

Department: Send Outs

Phone Number: (206) 987-2563

Reference Range

Age % Activity
0 - 1 month 15 - 150
1 month - 6 month 21 - 150
6 month - 1 year 36 - 150
1 year - Adult 60 - 150

 

Methodology

Method: Stago STA Clottable Factor assays

Analytical Volume:

Limitations:

CPT Codes

85250

Send Out Instructions

Reference Test Name: Factor IX Activity
Reference Test Number: F9
Instructions: See protocol for STAT send outs. If routine, send out Monday through Friday with the UW courier. If STAT, Delivery Express on dry ice to HMC GWH 47.