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Test Code FACTOR 9 Factor 9 Assay

Important Note

Method is affected by hemolysis and improper specimen handling. Patients on Direct Thrombin Inhibitors (Novastan, argatroban, bivalirudin) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations

Clinical System Name

Factor 9 Assay

Synonyms

Factor 9 Activity
Factor IX Activity
Factor IX Assay

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within one hour of collection.

Processing Instructions

Deliver blood to Core Coag bench. Coag Technologist will process.

 

Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device. 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

 

 

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

85250

Methodology

Method: Stago STA Clottable Factor assays

Analytical Volume:

Limitations:

Reference Range

Age % Activity
0 - 1 month 15 - 150
1 month - 6 month 21 - 150
6 month - 1 year 36 - 150
1 year - Adult 60 - 150

 

Send Out Instructions

Reference Test Name: Factor IX Activity
Reference Test Number: F9
Instructions: See protocol for STAT send outs. If routine, send out Monday through Friday with the UW courier. If STAT, Delivery Express on dry ice to HMC GWH 47.