Test Code FACTOR 9 Factor 9 Assay
Clinical System Name
Factor 9 Assay
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
Specimen Type | Temperature | Time |
---|---|---|
Citrated platelet-poor plasma | Room temp | N |
Refrigerated | N | |
Frozen | Y |
Performing Laboratory
Harborview Medical Center
Clinical Coagulation Lab
325 9th Ave
Room GWH 47
Seattle, WA 98195
Phone Number: (206) 744-3128
Department
Department: Send Outs
Phone Number: (206) 987-2563
Synonyms
Factor 9 Activity
Factor IX Activity
Factor IX Assay
Availability
STAT | Performed | TAT |
---|---|---|
N | M - F | 1 - 3 d |
Methodology
Method: Electromagnetic mechanical clot detection assay by STA-R MAX
Analytical Volume: 400 uL plasma
Limitations: Method is affected by henolysis, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Patients on Direct Thrombin Inhibitors (Novastan, argatroban, bivalirudin) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.
Reference Range
Age | % Activity |
0 - 1 month | 15 - 150 |
1 month - 6 month | 21 - 150 |
6 month - 1 year | 36 - 150 |
1 year - Adult | 60 - 150 |
CPT Codes
85250
Send Out Instructions
Reference Test Name: | Factor IX Activity |
Reference Test Number: | F9 |
Instructions: | See protocol for STAT send outs. If routine, send out Monday through Friday with the UW courier. If STAT, Delivery Express on dry ice to HMC GWH 47. |