Test Code FACTOR 9 Factor 9 Assay
Clinical System Name
Factor 9 Assay
Synonyms
Factor 9 Activity
Factor IX Activity
Factor IX Assay
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (3) 1.8 mL or (1) 2.7 mL
Minimum Vol: (2) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within ONE hour of collection.
Processing Instructions
Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: Core 6 Freezer ( -70 C) Send Out rack.
Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within ONE hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make two aliquots of 0.6 mL, affix with a large Epic label and store in -70 freezer, Send Outs rack.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within ONE of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic label. Freeze two aliquots of 0.6 mL plasma in aliquot tubes at -70C.
Stability
Specimen Type | Temperature | Time |
---|---|---|
Citrated platelet-poor plasma | Room temp | N |
Refrigerated | N | |
Frozen | Y |
Availability
STAT | Performed | TAT |
---|---|---|
N | M - F | 1 - 3 d |
Performing Laboratory
Harborview Medical Center
Clinical Coagulation Lab
325 9th Ave
Room GWH 47
Seattle, WA 98195
Phone Number: (206) 744-3128
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85250
Methodology
Method: Stago STA Clottable Factor assays
Analytical Volume:
Limitations:
Reference Range
Age | % Activity |
0 - 1 month | 15 - 150 |
1 month - 6 month | 21 - 150 |
6 month - 1 year | 36 - 150 |
1 year - Adult | 60 - 150 |
Send Out Instructions
Reference Test Name: | Factor IX Activity |
Reference Test Number: | F9 |
Instructions: | See protocol for STAT send outs. If routine, send out Monday through Friday with the UW courier. If STAT, Delivery Express on dry ice to HMC GWH 47. |