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Test Code FACTOR X Factor 10 Assay

Important Note

Don't confuse Factor X with Anti Factor XA (Low Molecular Weight Heparin). Patients on hirudin (Refludan) and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Factor 10 Assay

Synonyms

Factor X

 

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the  collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to: Clotted, Hemolyzed, Insufficient quantity, and Improper collection.

Spin: N

Aliquot: N

Temp: RT

Storage location: Core 6 Freezer ( -70 C) Send Outs rack.

 

Note: Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within  1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots  0.6 mL, affix large label and store in -70 freezer, Send Out rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N Drawn daily, Performed M - F at HMC Coag Lab 1 - 3 d

 

Performing Laboratory

University of Washington

Dept. of Laboratory Medicine

Harborview Medical Center Coag Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

 

 

Reference Range

Units: %
Female Male
Age Range Age Range
0 - 29d 19 - 150 0 - 29d 19 - 150
1m - 2m 31 - 150 1m - 2m 31 - 150
3m - 5m 35 - 150 3m - 5m 35 - 150
6m - 11m 38 - 150 6m - 11m 38 - 150
1y - 50 - 150 1y - 50 - 150

 

Methodology

Method: Stago STA Clottable Factor Assays

Analytical Volume: 1.0 mL citrate plasma

Limitations:

CPT Codes

85260

 

Send Out Instructions

Reference Test Name: Factor X Activity
Reference Test Number: F10
Instructions: Send out Monday - Friday with the UW courier.