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HelpTest Code FE-URINE Hemosiderin - Urine
Clinical System Name
Urine Iron Stain
Sample Requirements
Specimen: Random Urine
Container(s): Sterile Screw-Capped Container
Preferred Vol: 60 mL
Minimum Vol: 60 mL
Note: Specimen can be collected over 4 hours. Specimen should be sent to the lab soon after collection to avoid cell lysis. Deliver specimen to Urinalysis for initial processing. Actual staining for iron will be done by the SCH Histology department.
Processing Instructions
Reject due to: Specimens in preservatives, Insufficient quantity, incorrect storage temperature, age of specimen
Spin: N
Aliquot: Y (Done by Core Department)
Storage location: Core 5 Fridge
Off-site collection: Specimen should be sent to the lab within 1 hr after collection to avoid cell lysis. Send refrigerated.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | RT |
≤ 1 h |
| Urine | 2-8 C |
≤ 24 h |
| Urine | -20 C or -70 C |
≤ 7 d |
* Stability taken from http://ltd.aruplab.com/Tests/Pub/0020222
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F | 2-3 d |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
83070
Methodology
Method: Prussian Blue stain/microscopy
Analytical Volume: 12 mL
Reference Range
Hemosiderin not present
Description
During intravascular hemolysis plasma haptoglobin may be exhausted. Any free hemoglobin will then be filtered through the glomerulus. Some of the hemoglobin is reabsorbed into the tubular cells where the iron is removed and deposited within the cell as hemosiderin and ferritin. The hemosiderin granules may be found free, in epithelial cells or occasionally they may be seen in casts in the urine sediment.