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Test Code FE-URINE Hemosiderin - Urine

Clinical System Name

Urine Iron Stain


During intravascular hemolysis plasma haptoglobin may be exhausted. Any free hemoglobin will then be filtered through the glomerulus. Some of the hemoglobin is reabsorbed into the tubular cells where the iron is removed and deposited within the cell as hemosiderin and ferritin. The hemosiderin granules may be found free, in epithelial cells or occasionally they may be seen in casts in the urine sediment.

Sample Requirements

Specimen: Random Urine

Container(s): Sterile Screw-Capped Container

Preferred Vol: 60 mL

Minimum Vol: 60 mL


Note: Specimen can be collected over 4 hours. Specimen should be sent to the lab soon after collection to avoid cell lysis. Deliver specimen to Urinalysis for initial processing. Actual staining for iron will be done by the SCH Histology department.

Processing Instructions

Reject due to: Specimens in preservatives, Age/temperature of specimen and transport.

Spin: N

Aliquot: Y (Done by Histology Department)

Temp: 2-8oC

Storage location: Histology Department


Off-site collection: Specimen should be sent to the lab within 1 hr after collection to avoid cell lysis. Send  refrigerated.



Specimen Type Temperature Time
Urine RT

1 H

Urine 2-8oC

 24 H

Urine -20oC or -70oC

 7 D


* Stability taken from


STAT Performed TAT
N M-F 2-3 D


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617

Reference Range

Hemosiderin not present


Method:  Prussian Blue stain/microscopy

Analytical Volume:  12 mL

CPT Codes