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FIP1L1-PDGFRA fusion by FISH

Important Note

This test is under Utilization Management therefore must be ordered as a MISCELLANEOUS GENETIC TEST. All requests are reviewed and must be approved by a pathologist as part of the lab stewardship program.

Clinical System Name

Miscellaneous Genetic Test

Sample Requirements

Specimen: Whole Blood or Bone Marrow

Container(s): Lavender/EDTA or Dark Green/Sodium Heparin

Preferred Vol: 3.0 mL Whole Blood or 2.0 mL Bone Marrow

Minimum Vol: 2.0 mL Whole Blood or 1.0 mL Bone Marrow

 

Specimen: Solid Tumor (fresh):

  • Obtain sample (0.15-2.0cm3) under sterile conditions and place in transport media
  • Keep refrigerated.

Solid Fumor (FFPE/Formalin-Fixed, Paraffin-Embedded):

  • Provide an unstained slide (4μm thick section) per probe of IFISH and a corresponding H&E stained slide.
  • Do not send specimens that have been exposed to decalcifying agents.
  • Mail slides in a protected mailing container.

Contact pathology for assistance with submitting tissue.

 

Note: Record specimen type and date/time of collection on label.

 

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: RT

Storage Location: Affix large Cerner label to vacutainer. Store sample in room temp Send Outs rack.

 

Off-site Collection: Do not draw after hours and on weekends. DO NOT SPIN. Store and transport room temperature.

Stability

Specimen Type Temperature Time
Whole Blood or Bone Marrow Room temp 3 d
  Refrigerated 3 d
  Frozen N

 

Availability

STAT Performed TAT
Y   7 - 14 d

 

Performing Laboratory

University of Washington Medical Center

Cytogenetics and Genomics Laboratory

1959 NE Pacific Street, Room NW-125

Seattle, WA 98195

 

Phone: (206) 598-4488

Department

Department: Send Outs/Genetic

Phone: (206) 987-2563

 

Methodology

Method: Fluorescence In Situ Hybridization (FISH)

Analytical Volume: 2.0 Whole Blood or 1.0 mL Bone Marrow

Limitations:

Reference Range

Interpretive report provided.

Send Out Instructions

Reference Test Name: FIP1L1-PDGFRA fusion by FISH
Reference Test Number: 423
Instructions: Send sample Monday through Friday with the UW courier.