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Test Code Fungitell 1,3-Beta-D-Glucan (Fungitell), Serum


Specimen Required


Container/Tube: Serum gel (red top tube is not acceptable)

Specimen Volume: 1 mL

Collection Instructions:

1. Avoid exposure of specimen to atmosphere to prevent environmental contamination of the sample.

2. Centrifuge and send entire specimen in original collection tube. Do not aliquot or open tube.


Seattle Children's Hospital Note:

Gold Microtainers are not acceptable for this test. Collect 1.0 mL (Min. 0.5 mL) in a Gold Macrotainer.

Useful For

Aiding in the diagnosis of invasive fungal infections caused by various fungi, including Aspergillus species, Fusarium species, Candida species, and Pneumocystis jirovecii, among others

Method Name

Protease Zymogen-Based Colorimetric Assay

Reporting Name

(1, 3) Beta-D-Glucan (Fungitell), S
Seattle Children's Hospital Note:

SCH Clinical System Name: Fungitell (Beta-D-Glucan) Serum

Specimen Type

Serum SST

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Refrigerated (preferred) 14 days SERUM GEL TUBE
  Frozen  30 days SERUM GEL TUBE

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Finger/heel sticks Reject

Reference Values

Fungitell quantitative value:

<60 pg/mL

 

Fungitell qualitative result:

Negative

 

Reference values apply to all ages.

Method Description

The Fungitell assay measures levels of (1,3)-beta-D-glucan (BDG). The assay is based upon a modification of the limulus amebocyte lysate pathway. The Fungitell reagent is modified to eliminate factor C and, thus, to only react to BDG through the factor G-mediated side of the pathway. BDG activates factor G, a serine protease zymogen. The activated factor G converts the inactive proclotting enzyme to the active clotting enzyme, which in turn cleaves pNA from the chromogenic peptide substrate, Boc-Leu-Gly-Arg-pNA, creating a chromophore that absorbs at 405 nm. The Fungitell kinetic assay is based upon the determination of the rate of optical density increase produced by a sample. This rate is interpreted against a standard curve to produce estimates of BDG concentration in the sample.(Package insert: Assay for [(1,3]-Beta-D-Glucan in Serum. Associates of Cape Cod Inc; 06/13/2023)

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87449

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SFUNG (1, 3) Beta-D-Glucan (Fungitell), S 42176-8

 

Result ID Test Result Name Result LOINC Value
603615 Fungitell Quantitative Value 42176-8
603616 Fungitell Qualitative Result 93812-6

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Microbiology Test Request (T244)