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HelpTest Code GENT ; GENT TR ; GENT PK Gentamicin
Clinical System Name
Gentamicin (Random, Peak, Trough)
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red, Lavender/EDTA, or Lavender/EDTA microtainer
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Note on the requisition whether the specimen is peak, trough, or random. Draw peak level ½ hour after the end of a ½ hour infusion or 1 hour post IM injection. Draw trough level ½ hour before the next dose. Only Random level is available STAT.
Processing Instructions
Reject due to: Age of specimen
Spin: Y
Aliquot: Y
Storage location: Core 5 Chemistry Refrigerator
Off-site collection: Note on the requisition whether the specimen is peak, trough or random. Draw peak level ½ hour after the end of a ½ hour infusion or 1 hour post IM injection. Draw trough level ½ hour before the next dose. Only Random Level is available stat. Specimen should be centrifuged within 1 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum or Plasma | RT |
≤ 2 h |
| Serum or Plasma | 2-8 C |
≤ 7 d |
| Serum or Plasma | -20 C or -70 C |
≤ 14 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y (Random Level Only) | 24/7 | 1 H |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
80170
Methodology
Method: Two Point Rate method by Vitros 4600
Analytical Volume: 0.02 mL + 0.03 mL dead space
Reference Range
| All Ages | mcg/mL |
|
Peak (Therapeutic) |
4 - 12 |
| Trough | 0 - 2 |
| Toxic |
≥ 12 |
| Neonatal Levels and Suggested Dosing | |
| Trough level taken at 24 hours: | Suggested dosing interval: |
| ≤ 1 mcg/mL | 24 hours |
| 1.1 to 2.3 mcg/mL | 36 hours |
| 2.4 to 3.2 mcg/mL | 48 hours |
Critical Values
≥ 12 mcg/mL