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Test Code Gastrin Gastrin, Serum

Reporting Name

Gastrin, S

Useful For

Investigation of patients with achlorhydria or pernicious anemia


Investigation of patients suspected of having Zollinger-Ellison syndrome


Diagnosis of gastrinoma

Specimen Required

Patient Preparation:

1. Fasting (8 hours) required

2. For 12 hours before this test do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. If multiple specimens are drawn, submit each vial under a separate order.

2. Label specimens with corresponding draw time.

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  24 hours

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.-12 a.m.

Saturday; 6 a.m.-6 p.m.

Seattle Children's Hospital Note:

TAT: 3 - 5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Automated Chemiluminescent Immunometric Assay

Method Description

The Immulite 2000 Gastrin assay is a chemiluminescent, enzyme-labeled immunometric assay based on a ligand-labeled murine monoclonal capture antibody specific for gastrin and separation by antiligand-coated solid phase. The patient sample along with the ligand-labeled, antigastrin monoclonal antibody, an alkaline phosphatase-conjugated rabbit polyclonal antigastrin antibody, and an alkaline phosphatase-conjugated murine monoclonal antigastrin antibody are simultaneously incubated in the presence of the immobilized antiligand bead in a reaction tube. During the 60-minute incubation, gastrin molecules in the sample form antibody sandwich complexes that, in turn, bind to antiligand on the solid phase. Unbound conjugate is then removed by a centrifugal wash, after which luminogenic substrate is added and the reaction tube is incubated for an additional 5 minutes. The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of this intermediate results in the sustained emission of light. The bound complex and, thus, also the photon output, as measured by the luminometer is proportional to the concentration of gastrin in the sample.(Instruction manual: Immulite 2000 Gastrin. Siemens Medical Solutions Diagnostics, 5210 Pacific Concourse Drive, Los Angeles, CA 900455-6900. PIL2KGA-15, 2008-11-14)

Reference Values

<100 pg/mL

There is no evidence that fasting serum gastrin levels differ between adults and children. Although 8-hour fasts are difficult or impossible to enforce in small children, serum gastrin levels after shorter fasting periods (3-8 hours) may be 50% to 60% higher than the 8-hour fasting value.


For SI unit Reference Values, see

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
GAST Gastrin, S 2333-3


Result ID Test Result Name Result LOINC Value
GAST Gastrin, S 2333-3

Reject Due To


Mild OK; Gross reject


Mild OK; Gross OK




Nonfasting specimens


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Oncology Test Request Form (T729) (

Benign Hematology Test Request Form (T755) (