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Test Code HTL12 Human T-Cell Lymphotropic Virus Types I and II Antibody Screen with Confirmation, Serum

Reporting Name

HTLV-I/-II Ab Screen, S

Useful For

Qualitative detection of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II)-specific antibodies with confirmation and differentiation between HTLV-I and HTLV-II infection


This test should not be used to screen blood, human cells, tissues, or solid-organ donors.


This test is not intended for use on cord blood specimens.

Ordering Guidance

This test is for serum specimens only. For spinal fluid specimens, order HTLVC / Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Screen with Confirmation, Spinal Fluid.

Necessary Information

Date of collection is required.

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.

Specimen Type


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 28 days
  Refrigerated  7 days

Day(s) Performed

Monday, Tuesday, Thursday, Friday

Seattle Children's Hospital Note:

TAT: 4 - 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Enzyme Immunoassay (EIA)

Method Description

The Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay in which the solid phase (microwells) is coated with a purified human T-cell lymphotropic virus types I (HTLV-I) viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen. With the addition of a diluted test sample containing antibodies to either HTLV-I or HTLV-II, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated and the well is washed with buffer. Subsequently, antihuman immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added, which binds the antibody-antigen complex during a second incubation. Following a wash and incubation with TMB (tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution, which changes the color to yellow. The amount of HTLV-I / HTLV-II specific antibodies present in the sample is proportional to the color intensity.(Package insert: Avioq HTLV-I/II Microelisa System. Avioq, Inc; 05/2019)

Reference Values


Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


86689 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HTLVI HTLV-I/-II Ab Screen, S 29901-6


Result ID Test Result Name Result LOINC Value
9539 HTLV-I/-II Ab Screen, S 29901-6

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HTLVL HTLV-I/-II Ab Confirmation, S Yes No

Testing Algorithm

If the human T-cell lymphotropic virus types I and II (HTLV-I/-II) antibody screen is reactive, then HTLV-I/-II antibody confirmation by line immunoassay will be performed at an additional charge.

Report Available

1 to 4 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-treated or precipitated specimens Reject


If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.