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Test Code HTL12 Human T-Cell Lymphotropic Virus Types I and II (HTLV-I/-II) Antibody Screen with Confirmation, Serum

Reporting Name

HTLV-I/-II Ab Screen, S

Useful For

Qualitative detection of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II)-specific antibodies with confirmation and differentiation between HTLV-I and HTLV-II infection

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1 mL

Specimen Type


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 28 days
  Refrigerated  7 days

Day(s) and Time(s) Performed

Monday through Friday; varies

Seattle Children's Hospital Note:

TAT: 4 - 6 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme Immunoassay (EIA)

Method Description

The Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay in which the solid phase (microwells) is coated with a purified HTLV-I viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen. With the addition of a diluted test sample containing antibodies to either HTLV-I or HTLV-II, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated and the well is washed with buffer. Subsequently, antihuman immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added, which binds the antibody-antigen complex during a second incubation. Following a wash and incubation with TMB (tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution, which changes the color to yellow. The amount of antibody present in the sample is proportional to color development.(Package insert: Avioq HTLV-I/II Microelisa System, Avioq, Inc., Research Triangle Park, NC, June 2015)

Reference Values


Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


86689-Confirmation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HTLVI HTLV-I/-II Ab Screen, S 29901-6


Result ID Test Result Name Result LOINC Value
9539 HTLV-I/-II Ab Screen, S 29901-6

Testing Algorithm

If HTLV-I/-II antibody by EIA is reactive, then HTLV-I/-II antibody confirmation by line immunoassay will be performed at an additional charge.

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject


Mild OK; Gross reject



Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HTLVL HTLV-I/-II Ab Confirmation, S Yes No