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Test Code Hep D Ab Hepatitis D Virus Total Antibodies, Serum


Necessary Information


Date of draw is required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions.

2. Pour off serum into aliquot tube.


Useful For

Detection of hepatitis D virus (HDV)-specific total antibodies (combined IgG and IgM) in human serum

 

Diagnosis of concurrent HDV infection in patients with acute hepatitis B virus (HBV) infection (acute coinfection), chronic HBV infection (chronic coinfection), or acute exacerbation of known chronic HBV infection (HDV superinfection)

Special Instructions

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

HDV Total Ab, S

Specimen Type

Serum

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  30 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

Plasma

Reference Values

Negative

Method Description

This test is performed using a competitive enzyme immunoassay in which hepatitis delta virus-specific antibodies (anti-HDV) compete with virus-specific polyclonal IgG antibody that is labeled with peroxidase (HRP) for a fixed amount of recombinant HDV protein coated on the microplate wells. Patient serum sample is added first to the microplate well, in which anti-HDV IgG and IgM antibodies will bind to the recombinant HDV protein coated in the well. After washing, a polyclonal anti-HDV-enzyme conjugate is added and allowed to bind to unbound recombinant HDV protein. After another wash, a chromogenic mixture is added as a substrate for the HRP enzymatic reaction. Concentration of the enzyme conjugate bound to the coated well is inversely proportional to the amount of HDV total antibodies present in the patient sample. The concentration of HDV total antibodies present is determined by comparing the calorimetric reaction signal to a calibrated cut-off signal value.(Package insert: HDV Ab, Rev.4 0115; International Immuno Diagnostics, Foster City, CA 94404 01/2015)

Day(s) and Time(s) Performed

Varies

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86692

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AHDV HDV Total Ab, S 40727-0

 

Result ID Test Result Name Result LOINC Value
9209 HDV Total Ab, S 40727-0