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Test Code LAB106 B-Type Natriuretic Peptide

Clinical System Name

B-type Natriuretic Peptide




Brain Natriuretic Peptide

Sample Requirements

Specimen:  Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL Microtainer; 1.0 mL Macrotainer


Notes : A Lavender top/EDTA Macrotainer must contain at least 1.0 mL.

Specimens drawn in glass tubes will be rejected.

Off site minimum volume is 2 EDTA Microtainers or 1 mL EDTA Macrotainer.

Processing Instructions

Reject due to: Insufficient Quantity, Wrong Sample Collection, Age of specimen, Specimens drawn in glass tubes.

Spin: N

Aliquot: N

Storage location: Core 5 Chemistry Refrigerator


Off-site collection: Specimen should be centrifuged within 7 hours of collection. Spin blood and transfer plasma to plastic tube and refrigerate or freeze. Method affected by prolonged exposure to RBCs.


Specimen Type Temperature Time
EDTA Plasma RT

≤ 7 h

EDTA Plasma 2-8 C

≤ 24 h

EDTA Plasma -20 C or -70 C

≤ 365 d



Specimen Type Temperature Time
EDTA Whole Blood RT

≤ 7 h

EDTA Whole Blood 2-8 C

≤ 7 h

  -20 C or -70 C N


STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    


Department: Core Chemistry

Phone Number206-987-2617 (Client Services)

CPT Codes



Method: Fluorescent Immunoassay (FIA) by Triage MeterPro

Analytical Volume: 0.25 mL EDTA Whole Blood or Plasma

Reference Range

 Age  pg/mL
0 - 7 d   0 - 170
> 7 d 0 - 41


BNP is a neurohormone synthesized primarily by ventricular cardiac myocytes. Increased plasma BNP levels have been observed in adults with congestive heart failure, ventricular hypertrophy, heart transplant rejection, and other conditions. It typically has been used as an aid in the diagnosis and assessment of severity of congestive heart failure, but studies have shown an association between heart transplant rejection and persistent elevated BNP levels, and a reduction in BNP levels with successful treatment of transplant rejection.