Sign in →

Test Code LAB1124 Plasminogen Activator Inhibitor Antigen, Plasma


Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial (polypropylene preferred)

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot plasma into a plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.

Specimen Stability Information: Frozen 2 years

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Identification of heredity elevation or deficiency of plasminogen activator inhibitor type 1

 

Determination of risk for veno-occlusive disease associated with bone marrow transplantation

 

Differential diagnosis of impaired fibrinolysis

 

Prognostic marker of occurrence or recurrence of thrombosis

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

PAI-1 Ag, P
Seattle Children's Hospital Note:

SCH Clinical System Name: Plasminogen Activator Inhibitor

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

3.0-72.0 ng/mL

Method Description

Testing is performed on the Janus G3 liquid handler and BioTek microplate reader with Gen5 software using the Asserachrom PAI-1 kit. The method used is an enzyme-linked immunosorbent assay. Microtiter plate wells are coated with mouse monoclonal antihuman PAI-1 antibody, which captures the PAI-1 in the sample. Next, a second antibody (mouse monoclonal antihuman PAI-1) coupled with peroxidase binds to another antigenic site distant from the first antibody, forming the "sandwich.'' The bound enzyme peroxidase is then visualized based on its ability to produce a color reaction when exposed to ortho-phenylenediamine and hydrogen peroxide. The reaction is stopped by the addition of a strong acid (1M HCl). The intensity of the color produced is directly proportional to the PAI-1 concentration in the plasma sample.(Package insert: ASSERACHROM PAI-1. Diagnostica Stago S.A.S; 03/2015)

Day(s) Performed

Wednesday

Report Available

7 to 12 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85415

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PAI1 PAI-1 Ag, P 22758-7

 

Result ID Test Result Name Result LOINC Value
86083 PAI-1 Ag, P 22758-7