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Test Code LAB1232 Toxocara Antibody, IgG, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Aiding in the diagnosis of Toxocara infection

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Toxocara Ab, IgG, S
Seattle Children's Hospital Note:

SCH Clinical System Name: Toxocara Antibody

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated Reject

Reference Values

Negative

 

Reference values apply to all ages.

Method Description

Microtiter strip wells are precoated with synthetic Toxocara canis antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase-labeled protein A conjugate is added. This conjugate binds to the captured T canis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of T canis-specific antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Toxocara canis ELISA IgG Test Kit. Gold Standard Diagnostics; 07/11/2023)

Day(s) Performed

Tuesday, Thursday

Report Available

1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86682

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TOXCG Toxocara Ab, IgG, S 40674-4

 

Result ID Test Result Name Result LOINC Value
TOXCG Toxocara Ab, IgG, S 40674-4