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HelpTest Code LAB129 Thyroid Stimulating Hormone (TSH)
Clinical System Name
Thyroid Stimulating Hormone (TSH)
Synonyms
TSH
3rd generation assay
Sample Requirements
Specimen: Whole blood
Container(s): Gold SST, Red, Gold Microtainer, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, Lavender/EDTA
Preferred Vol: 2.0 mL
Minimum Vol: 1.0 mL
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2-8 C
Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.
Note: Minimum volume of 1.0 mL may be shared with TSH, T4, free T3, and free T4.
Off-site collection: Spin blood. Transfer serum/plasma to plastic aliquot container. Refrigerate.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| gel separator with serum/plasma | Refrigerated | 24 h |
| separated serum/plasma | Room temp | 2 h |
| Refrigerated | 7 d | |
| Frozen -20 C | 6 mo |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-Sa, day shift | 1-3 d |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem West
Phone Number: (206) 987-2617
CPT Codes
84443
Methodology
Method: Chemiluminescent microparticle immunoassay (CMIA) by Abbott Alinity i, third generation
Analytical Volume: 0.300 mL serum/plasma
Limitations:
Reference Range
| Age |
TSH (mcIU/mL) |
Critical High |
|---|---|---|
| 0 - <4 d | 0.5 - 18.2 | >38.5 |
| 4 d - <1 mo | 0.5 - 6.54 | >11.9 |
| 1 mo - <2 mo | 0.5 - 6.54 | |
| 2 mo - 19 yr | 0.47 - 4.17 | |
| >19 yr | 0.35 - 4.94 |
TSH transitioned platforms from Ortho Vitros 3600 to Abbott Alinity i on August 8, 2022.
There is a 25% negative bias with the new method.