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HelpTest Code LAB131 AST (SGOT)
Clinical System Name
AST (SGOT)
Synonyms
LAB131
AST (Aspartate Transaminase)
GOT
SGOT
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Test is affected by hemolysis. Send to lab promptly.
Processing Instructions
Reject due to: Marked hemolysis, age of specimen
Spin: Y
Aliquot: Y
Storage location: CORE 5 Refrigerator
Off-site collection: Avoid hemolysis. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
|
Serum or Plasma |
RT |
≤ 3 d |
|
Serum or Plasma |
2-4 C |
≤ 7 d |
|
Serum or Plasma |
-20 C or -70 C |
≤ 90 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Lab
Phone Number: 206-987-2617 (Client Services)
CPT Codes
84450
Methodology
Method: Spectrophotometric, Enzyme Rate by Vitros 4600
Analytical Volume: 0.02 mL + 0.03 mL dead space
Reference Range
| Age |
IU/L at 37°C |
|
0 - 1 Y |
3 - 74 |
|
1 - 7 Y |
28 - 66 |
| 7 - 13 Y |
23 - 50 |
| > 13 Y |
20 - 48 |
Note: Reference Range updated 4/4/2024
Description
Aspartate aminotransferase is present in high activity in heart, skeletal muscle, and liver. Increased serum AST activity commonly follows myocardial infarction, pulmonary emboli, skeletal muscle trauma, alcoholic cirrhosis, viral hepatitis, and drug-induced hepatitis.