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HelpTest Code LAB132 ALT
Clinical System Name
ALT (SGPT)
Synonyms
LAB132
ALT
Alanine Amino-Transferase (SGPT)
GPT
SGPT
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Processing Instructions
Reject due to: Age of Specimen
Spin: Y
Aliquot: Y
Storage location: Core 5 Refrigerator
Off-site collection: Specimen should be centrifuged within 2 hours of collection. Spin blood, and transfer serum or plasma to plastic tube and refrigerate.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum or Plasma | RT |
≤ 18 h |
| Serum or Plasma | 2-8 C |
≤ 7 d |
| Serum or Plasma | -20 C or -70 C | ≤ 3 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Lab Chemistry
Phone Number: 206-987-2617 (Client Services)
CPT Codes
84460
Methodology
Method: Spectrophotometric, Enzyme Rate by Vitros 4600
Analytical Volume: 0.02 mL + 0.03 mL dead space
Reference Range
| IU/L at 37C | |
| 0- 1 y | 5 - 50 |
| 1 y - 12 y | 5 - 31 |
| 12 y - 19 y (male) | 5 - 52 |
| 12 y - 19 y (female) | 5 - 38 |
| 19 y - adult (male) | 5 - 50 |
| 19 y - adult (female) | 5 - 35 |
Note: Reference ranges updated on 12/27/2018.
Description
Alanine aminotransferase is present in high activity in liver, skeletal muscle, heart and kidney. Serum ALT increases rapidly in liver cell necrosis, hepatitis, hepatic cirrhosis, liver tumors, obstructive jaundice, Reye's syndrome, extensive trauma to skeletal muscle, myositis, myocarditis, and myocardial infarction.