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Test Code LAB18 Lipid Panel

Important Note

Effective January 11, 2022, the National Institutes of Health (NIH) equation will replace the Friedewald equation for all lipid panels that report calculated low-density lipoprotein cholesterol (LDL).
The NIH calculation provides a more accurate LDL result than the Friedewald equation, especially in patients with low LDL concentrations (<70 mg/dL).

 

Patient should be fasting (at least 8 hours). For patients <2 years, fasting is 4 hours.

Clinical System Name

Lipid Panel

Synonyms

Cholesterol
HDL Cholesterol
LDL Cholesterol
Lipids, fasting
Triglycerides

LIP PRO

Lipid Profile

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note:  Test includes cholesterol, triglycerides, HDL and LDL (calculated).

 

Processing Instructions

Reject due to: Age of specimen, incorrect collection container

Spin: Y

Aliquot: Y

Storage location:  Core 5 Chemistry Refrigerator

 

Off-site collection:  Patient should be fasting. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.

Stability

Specimen Type Temperature Time
Serum or Plasma RT

≤ 3 h  
Serum or Plasma 2-8 C

≤ 3 d
Serum or Plasma -20 C or -70 C

≤ 21 d  

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)

CPT Codes

80061

Methodology

Method: Colorimetric method by Vitros 4600

Analytical Volume: 0.04 mL + 0.03 mL dead space

Reference Range

               Reference Ranges

Analyte (unit)

Age

Serum or Plasma

Cholesterol, fasting (mg/dL)

 

0 - 1 m

1 m - adult

50 - 120

125 - 200

Triglyceride, fasting (mg/dL)

All ages

60 - 135

HDL/Cholesterol (mg/dL)

0 - 6 y, Male

0 - 6 y, Female

6 y - 15 y

15 y - adult, Male

15 y - adult, Female

30 - 65

30 - 70

38 - 75

30 - 63

35 - 74

LDL/Cholesterol (mg/dL)

All ages, Male

All ages, Female

63 - 130

65 - 137

Description

NIH equation:  LDL-C = TC/0.948 – HDL-C/0.971 – (TG/8.56 + [TG x NonHDL-C]/2140 – TG2/16100) – 9.44

 

1.    Sampson M, Ling C, Sun Q, Harb R, Ashmaig M, Warnick R, Sethi A, Fleming JK, Otvos JD, Meeusen JW, Delaney SR, Jaffe AS, Shamburek R, Amar M, Remaley AT. A New Equation for Calculation of Low-Density Lipoprotein Cholesterol in Patients With Normolipidemia and/or Hypertriglyceridemia. JAMA Cardiol. 2020 Feb 26;5(5):1-9.
2.    Sathiyakumar V, Blumenthal RS, Elshazly MB. New information on accuracy of LDL-C estimation. American College of Cardiology. March 20, 2020. Accessed August 14, 2020. https://www.acc.org/latest-in-cardiology/articles/2020/03/19/16/00/new-information-on-accuracy-of-ldl-c-estimation