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Test Code LAB1800 Constitutional FISH

Important Note

This test is for Constitutional (germline) FISH test requests only. 

For other FISH testing, please see:

  • Cytogenetics Family Study FISH for parental or other family member follow up FISH studies
  • Cytogenetics Oncology FISH for studies used in the diagnosis of leukemia and other hematologic disorders

Clinical System Name

Constitutional FISH

Synonyms

Cytogenetics FISH

Description

This is a targeted test performed on interphase or metaphase cells to detect constitutional deletions or duplications of chromosomal material in the region of interest. 

 

Sample Requirements

Specimen: Whole Blood

Container(s): Dark Green/Sodium Heparin (no serum separator)

Preferred Vol:  5.0 mL (3.0 mL for infants <24 months)

Minimum Vol:  3.0 mL (1.0 mL for infants <24 months); no micro collection

 

Note:  Keep sample at room temperature; deliver to the lab promptly.  Samples received after 3 pm will be set up the following day.

Note:  For patients who have had a whole blood transfusion, wait 10 days post transfusion to draw for Cytogenetics.  No wait time is necessary if the patient received red cells or plasma. 

 

For other acceptable specimen types for CONSTITUTIONAL FISH testing please call Cytogenetics Lab, 206-987-1008.

 

 

Processing Instructions

Reject due to: Collected in microtainer.

Spin: N

Aliquot: N

Temp: RT

Storage location:  Days: Transport specimen, requistion, and labels to 4th floor Cytogenetics (station #181). 

Eves/Nights: Store specimen, copy of requisition, and labels in the Cytogenetics box in CPA.

 

Off-site collection: Keep whole blood at room temperature. Transport to Children's Lab ASAP. Samples received after 3 pm will be set up the following day.

Stability

Temperature Time
Room temp 3 d (call laboratory if sample is older)
Refrigerated No
Frozen No

 

Availability

STAT Performed TAT
N Daily 1 - 2 w

 

Performing Laboratory

Seattle Children's Hospital

Department

Department: Cytogenetics Laboratory 

Phone number: 206-987-1008

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

 

Lab Genetic Counselor: LabGC@seattlechildrens.org

 

CPT Codes

Interphase FISH: 88271, 88275, 88291, 88230

Metaphase FISH: 88271, 88273, 88291, 88230

 

Call or email Lab Client Services for CPT codes for other FISH panels

Methodology

Method: Fluorescent in-situ hybridization

Requisition

Molecular Genetics & Cytogenetics Requisition

Clinical Utility

FISH can be used to confirm or rule out targeted chromosomal abnormalities.