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HelpTest Code LAB2005 NT-Pro B-Type Natriuretic Peptide, Serum
Necessary Information
Patient's age and sex are required.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Useful For
Aiding in the diagnosis of congestive heart failure using serum specimens
Testing Algorithm
For more information see Amyloidosis: Laboratory Approach to Diagnosis
Special Instructions
Method Name
Electrochemiluminescence Immunoassay
Reporting Name
NT-Pro BNP, SSeattle Children's Clinical System Name: N-TERMINAL PROBNP
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 365 days | |
| Refrigerated | 7 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Males
0-2 day: 321-11,987 pg/mL
3-11 day: 263-5918 pg/mL
12 day-1 month: Not applicable
2 month-1 year: 37-646 pg/mL
2 years: 39-413 pg/mL
3 years-6 years: 23-289 pg/mL
7 years-14 years: ≤157 pg/mL
15 years-18 years: ≤158 pg/mL
19-39 years: <79 pg/mL
40-44 years: ≤72 pg/mL
45-54 years: ≤87 pg/mL
55-64 years: ≤88 pg/mL
≥65 years: ≤540 pg/mL
Females
0-2 day: 321-11,987 pg/mL
3-11 day: 263-5918 pg/mL
12 day-1 month: Not applicable
2 month-1 year: 37-646 pg/mL
2 years: 39-413 pg/mL
3 years-6 years: 23-289 pg/mL
7 years-14 years: < or=157 pg/mL
15 years-18 years: ≤158 pg/mL
19-39 years: <160 pg/mL
40-44 years: ≤162 pg/mL
45-54 years: ≤141 pg/mL
55-64 years: ≤226 pg/mL
≥65 years: ≤540 pg/mL
Method Description
This is an automated, double-incubation sandwich assay. In the first incubation, antigen from the patient specimen reacts with biotinylated monoclonal sheep N-terminal pro brain natriuretic peptide (NT-proBNP) antibody and monoclonal NT-proBNP antibody labeled with ruthenium complex. During the second incubation, streptavidin-labeled microparticles are added, and the resulting complex is bound to the solid-phase via biotin-streptavidin interaction. The resulting reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of an electrode. Unbound substances are washed away. Voltage is then applied to the electrode, which induces chemiluminescent emission that is measured by a photomultiplier. Results are obtained by comparing this measurement against the calibration curve. The Roche E Modular NT-proBNP assay standardization is traceable to an internal Roche standard. Assigned values for calibrators are traceable to this standardization.
The Roche NT-proBNP assay is automated and more precise than the Biosite BNP assay used previously. In addition, in vitro NT-proBNP is more stable than BNP.(Package insert: ProBNP II, Roche Diagnostics; V 12.0. 07/2021)
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysTAT: 3 - 4 d
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83880
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PBNP1 | NT-Pro BNP, S | 83107-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| PBNP1 | NT-Pro BNP, S | 83107-3 |
Forms
If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.