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Test Code LAB23 Digoxin Level

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Collect in Gold SST, Gold Microtainer, or Red Top-Plain. Samples collected in heparin will be rejected.

Clinical System Name

Digoxin Level





Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Gold Microtainer, Red 

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL  


Note:  Draw specimen immediately before next dose (within 30 minutes).  Avoid hemolysis. A hemoglobin level of 300 mG/dl causes a bias of -0.6. Test is also affected if patient is hypertensive, uremic, or a neonate. There is some cross-reactivity to Digoxin-Like Immunoreactive Factors (DLIF). Digibind affects method. Method is not affected by moderate lipemia.

Processing Instructions

Reject due to: Age of specimen, incorrect collection container.

Spin: Y

Aliquot: Y

Storage location:  Core Chemistry Refrigerator


Off-site collection:  Draw specimen immediately before next dose (within 30 minutes). Avoid hemolysis. Specimen should be centrifuged within 2 hours of collection. Spin blood and transfer serum to plastic tube and refrigerate or freeze.


Specimen Type Temperature Time
Serum RT

≤ 8 h

Serum 2-8 C

≤ 7 d

Serum -20 C or -70 C

≤ 4 m


STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)

CPT Codes



Method:  Multiple Point Immuno-Rate method by Vitros 4600

Analytical Volume: 0.02 mL + 0.03 mL dead space


Method is affected by EDTA & hemolysis.

Reference Range

0.5 - 2.0 ng/mL



Digoxin is a widely prescribed cardiac glycoside indicated in the treatment of congestive heart failure and supraventricular arrhythmias. Digoxin measurements are used to monitor patient compliance and therapy, and to diagnose potential overdose.

Critical Values

> 2.5 ng/mL