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HelpTest Code LAB2333 Sex Hormone-Binding Globulin, Serum
Additional Codes
Sex Hormone Binding Globulin
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Diagnosis and follow-up of women with signs or symptoms of androgen excess (eg, polycystic ovarian syndrome and idiopathic hirsutism)
An adjunct in monitoring sex-steroid and antiandrogen therapy
An adjunct in the diagnosis of disorders of puberty
An adjunct in the diagnosis and follow-up of anorexia nervosa
An adjunct in the diagnosis of thyrotoxicosis (tissue marker of thyroid hormone excess)
A possible adjunct in diagnosis and follow-up of insulin resistance and cardiovascular and type 2 diabetes risk assessment, particularly in women
Method Name
Immunoenzymatic Assay
Reporting Name
Sex Hormone-Binding Globulin, SClinical System Name: Sex Hormone Binding Globulin
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 90 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
CHILDREN:
Males
|
Tanner Stages |
Mean Age |
Reference Interval (nmol/L) |
|
Stage I |
10.4 |
17-135 |
|
Stage II |
11.1 |
21-114 |
|
Stage III |
12.7 |
12-138 |
|
Stage IV |
14.5 |
7.7-67 |
|
Stage V |
14.2 |
3.9-40 |
Females
|
Tanner Stages |
Mean Age |
Reference Interval (nmol/L) |
|
Stage I |
10.5 |
16-182 |
|
Stage II |
10.9 |
24-121 |
|
Stage III |
12.5 |
18-87 |
|
Stage IV |
14 |
7.7-108 |
|
Stage V |
14.9 |
10-79 |
ADULTS:
Males
≥18 years: 13.3-89.5 nmol/L
Females
18-46 years: 18.2-135.5 nmol/L
47-91 years: post-menopausal: 16.8-125.2 nmol/L
Method Description
The Access SHBG (sex hormone-binding globulinSHBG) assay is a sequential 2-step immunoenzymatic (sandwich) assay. Patient sample is added to a reaction vessel along with paramagnetic particles coated with monoclonal anti-SHBG antibody and saline buffer with proteins. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. A second monoclonal anti-SHBG antibody conjugated to alkaline phosphatase is added to the reaction vessel. After the second incubation in the reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Then, the chemiluminescent substrate is added to the vessel, and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of SHBG in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.(Package insert: Access SHBG Sex Hormone-Binding Globulin, A48617. Beckman Coulter, Inc; 04/2020)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
84270
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SHBG1 | Sex Hormone-Binding Globulin, S | 13967-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SHBG1 | Sex Hormone-Binding Globulin, S | 13967-5 |
Day(s) Performed
Monday through Saturday