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Test Code LAB2394 Chlam trachomatis and N. gonorrhoeae PCR

Important Note

For sexual assault cases and urgent / critical cases, please contact the Micro lab directly at 7-2585

Infectious

Synonyms

Chlamydia trachomatis and Neisseria gonorrhoeae PCR

Sample Requirements

Specimen: Dirty Catch Urine*, Endocervical, Vaginal, Throat, Rectal


NOTE: For non-FDA approved specimens, please order Chlamydia trachomatis and Neisseria gonorrhoeae, Miscellaneous Sites (Eye) or Chlam trachomatis and GC Aptima UW PCR (Urethral, Penile). Collect samples using the Apitma Unisex or Aptima Multitest Collection Kit.

Eye samples will be sent to Mayo Clinic Laboratory for testing. Include note for ‘CT NG PCR (Test Code #MCTGC) at Mayo Clinic’ in the order comments.

Penile and Urethral samples will be sent to UWMC Laboratory for testing.

 

Container:

  • Sterile Container for all Urines
  • CT/NG swabs for all in house PCR testing
    • GeneXpert Vaginal Endocervical Collection kit
    • Xpert Swab Specimen Collection Kit
  • If sample is bloody collect instead:
    • "Aptima Unisex Collection kit" for Endovervical or Urethral sites.
    • "Aptima Multitest Collection kit" for Vaginal, Penile, Throat, or Rectal Sites
  • eSwabs are only accepted for CT GC PCR ordered through the Sexual Assault PowerPlan

Preferred Vol: 10mL for Urine

Minimum Vol: 7mL for Urine**

 

Please consult the Photo Swab Guide to determine which swab to collect.

Please see Sexual Assault Photo Swab Guide for forensic evaluations.

 

*Dirty Catch urine is the first stream of the first morning void (for maximum sensitivity), or the first stream at least 1 hour after the last void. Patient should not perform any genital cleaning before collection. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted at least one hour since the last void. Clean catch urines are unacceptable for testing and will be REJECTED.

 

**Urine samples with suboptimal volumes (between 2mL - 7mL) will be sent to the University of Washington for testing.  Send urine to lab in sterile container.  Urine samples of less than 2mL are unacceptable and will be REJECTED.

 

Bloody swabs/urine samples will be sent to University of Washington for testing.  Send bloody urine to lab in sterile container.  Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit. Call Microbiology (206-987-2585) for APTIMA Kits.

Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.

 

Processing Instructions

CPA: Send sample to Microbiology for processing.

Regional Clinic collection:  Please consult the Photo Swab Guide to determine which swab to collect. Send to SCH Main Campus for testing. Refrigerate before and during courier transport. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Stability

Urine
Temp Time
Room temp 24 h
Refrigerated 1 w
Frozen N

 

Xpert Vaginal/Endocervical Swabs
Temp Time
Room temp 60 d
Refrigerated 60 d
Frozen N

 

Aptima Multitest Swab Specimen Collection Kit
Temp Time
Room temp 30 d
Refrigerated 30 d
Frozen N

 

Aptima Unisex Swab Specimen Collection Kit
Temp Time
Room temp 30 d
Refrigerated 30 d
Frozen N

Availability

Procedure Performed Turn Around Time (TAT)
In-House PCR (Cepheid) 24/7 3 h
APTIMA PCR (for bloody samples sent to UW for testing) 24/7

3 - 5 d

Performing Laboratory

Harborview Medical Center

Clinical Microbiology Laboratory

325 9th Avenue, Room GWH 47

Seattle, WA 98104

 

Phone Number: Microbiology Lab - (206) 744-5858; UW Client Support Services - (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

87491

87591

Clincal System Name

Chlam trachomatis and N. gonorrhoeae PCR

Description

This includes nucleic acid amplification for Chlamydia trachomatis and Neisseria gonorrhoaea with amplified probe technique.

Reporting Information

Final Report (Cepheid): 3 hours

Final Report (Aptima): 3-5 days

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf