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HelpTest Code LAB2399 Cortisol, Saliva
Additional Codes
Cort Sal
Reporting Name
Cortisol, SalivaSCH Clinical System Name: Cortisol Level, Saliva
Useful For
Screening for Cushing syndrome
Diagnosis of Cushing syndrome in patients presenting with symptoms or signs suggestive of the disease
Necessary Information
Collection time is required.
Specimen Required
If multiple specimens are collected, submit each Salivette under a separate order number.
Patient Preparation:
1. Patient should not brush teeth, eat, drink, or take any oral medication for at least 60 minutes before specimen collection.
2. Have patient rinse mouth thoroughly with water for 1-5 seconds. Wait 10 minutes before collecting the specimen to avoid contamination of the saliva by interfering substance.
Supplies: Cortisol, Saliva Collection Kit (T514)
Container/Tube: Sarstedt Salivette
Specimen Volume: 1.5 mL
Collection Instructions:
1. Provide patient with a Saliva Collection Kit (Salivette) containing the Cortisol - Saliva Collection Instructions and ask them to follow the instructions as written.
2. Instruct patient to collect specimen between 11 p.m. and midnight and record collection time on the Cortisol - Saliva Collection Instructions sheet.
3. Instruct patient to return Cortisol - Saliva Collection Instructions with the appropriately labeled Salivette to the laboratory.
Additional Information: Reference values are also available for an 8 a.m. (7 a.m.-9 a.m.) or a 4 p.m. (3 p.m.-5 p.m.) collection, however, the 11 p.m. to midnight collection is preferred.
SARSTEDT Salivette preferred, other salivettes (such as Oasis Diagnostics) are acceptable.
If multiple samples collected on different days are received in lab, each sample needs its own financial encounter for billing purposes.
Specimen Type
SalivaSpecimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Saliva | Refrigerated (preferred) | 28 days |
| Frozen | 60 days | |
| Ambient | 28 days |
Day(s) Performed
Monday through Friday
TAT: 4 - 6 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
Isotopically labeled cortisol and cortisone are added to the sample as internal standards. Cortisol, cortisone, and coinciding internal standards are extracted from the specimens using online turbulent flow chromatography, which is followed by liquid chromatography tandem mass spectrometry analysis. Although cortisone is not reported, it is used to verify the cortisol value and screen for possible contamination from exogenous sources.(Unpublished Mayo method)
Reference Values
7 a.m.-9 a.m.: 100-750 ng/dL
3 p.m.-5 p.m.: <401 ng/dL
11 p.m.-midnight: <100 ng/dL
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82533
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SALCT | Cortisol, Saliva | 2142-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 84225 | Cortisol, Saliva | 2142-8 |
| 23612 | AM Cortisol | 58674-3 |
| 23613 | PM Cortisol | 58668-5 |
| 23614 | Midnight Cortisol | 58642-0 |
Report Available
2 to 5 daysReject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Special Instructions
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Renal Diagnostics Test Request (T830)
Aliases: Nighttime Salivary Cortisol; Salivary Cortisol; Salivary Cushings; Salivary Hydrocortisone