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HelpTest Code LAB2424 Pyruvate, CSF
Clinical System Name
CSF Pyruvate
Sample Requirements
Specimen: Cerebrospinal fluid
Container(s): Sterile Specimen Container
Preferred Vol: 1 mL
Minimum Vol: 1 mL
Note: Place the sample on ice and deliver to the lab within 10 minutes of collection. Note if patient was crying, or if collection was difficult.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: - 20 C
Storage location: Deliver to CORE within 2 min of receipt. A protein-free filtrate must be made by CORE as soon as possible.
CORE: Add 0.5 mL CSF to 1.0 mL cold 6% perchloric acid and mix by vortexing. Centrifuge and transfer supernate to plastic tube. Store in CPA Freezer, Fz5 rack.
Off-site collection: Prepare protein-free filtrate: Add 0.5 mL CSF to 1.0 mL cold 6% perchloric acid (PCA) and mix by vortexing. Centrifuge and transfer supernate to plastic tube. Send frozen.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| protein-free filtrate, CSF | Room temp | |
| Refrigerated | 4 d | |
| Frozen | 4 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M, W, F | 1-4 d |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
84210
Methodology
Method: Enzymatic Rate Reaction by Vitros 4600
Analytical Volume: 0.5 mL
Limitations:
Reference Range
All ages: 0.06 - 0.19 mmol/L
Description
CSF lactate and pyruvate are measured to calculate a lactate-to-pyruvate ratio in the investigation of possible mitochondrial disease.