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HelpTest Code LAB2451 Rapid Stool Infectious Diarrhea PCR Panel
Infectious
Clinical System Name
Rapid Stool Infectious Diarrhea PCR Pnl
Synonyms
Stool testing
Stool PCR
Rapid Stool PCR
Stool Infectious Testing
Diarrhea
Stool Viral Testing
Stool Culture
Rotavirus
Giardia
Norovirus
Adenovirus
LAB2451
Sample Requirements
Specimen: Liquid or unformed stool collected in a clean leak-proof container is the optimal sample type.
Rectal swabs are not optimal. In rare situations, exceptions may be made by contacting the Microbiology Lab 7-2585. Swab samples should be inserted at a distance sufficient to have visible stool on the swab (usually requires insertion of swab several centimeters into the rectum). Use the white capped E Swab System for collection.
Formed stool, stool in a fixative or stool received in a diaper are not acceptable specimen types. These specimens will be rejected for testing. For formed stools in which diagnostic testing is indicated, providers should consider Stool Culture [LAB223].
Container: Clean leak-proof container
Preferred volume: 1 gram or ml
Minimum volume: 0.5 gram or ml
Processing Instructions
CPA: Send stool to Microbiology ASAP. If sample is shared and only one tube, syringe, or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology. Please call Microbiology with sample related questions.
Off-Site Locations: This testing is only performed at SCH Microbiology. For satellite locations, the sample should be refrigerated prior to and during transport to the SCH Micro lab. Refer to stability requirements.
Stability
| Specimen | Temp | Time |
| Fresh Stool | Room Temp | 2 hours |
| Refrigerated | ≤24 hours | |
| Frozen (-20C) | 4 days |
Availability
| Procedure | Performed | Turn Around Time (TAT) |
| FilmArray PCR | 24/7 | 2 hours |
Performing Laboratory
Seattle Children's Laboratory
Department
Microbiology
CPT Codes
87507
Methodology
Molecular method by BioFire FilmArray (Film Array)
Reference Range
Negative
Note: Samples that are PCR positive for the following bacterial pathogens will be plated for culture at no additional charge (for epidemiological purposes and/or susceptibility testing).
Plesiomonas
Salmonella
Yersinia
E coli O157
STEC non-O157
Shigella
Vibrio cholera, V parahaemolyticus, and V vulnificus
Description
The FilmArray Gastointestinal Panel (FA GI) is a multiplexed nucleic acid test designed for simultaneous qualitative detection and identification of multiple pathogens associated with gastrointestinal infection.
The infectious agents detected by this panel include:
Campylobacter (jejuni, coli, and upsaliensis)
Plesiomonas
Salmonella
Yersinia enterocolitica
E coli (enterotoxigenic E coli) ETEC
Shiga-like toxin-producing E coli (STEC) stx1/stx2
E coli O157
Shigella sp
Vibrio parahaemolyticus, vulnificus, and cholera
Cryptosporidium sp
Cyclospora sp
Entamoeba histolytica
Giardia
Adenovirus
Astrovirus
Norovirus
Rotavirus
Sapovirus
This test is NOT appropriate for test of cure. Following treatment, the patient can be retested by submitting a Stool Culture, or appropriate EIA testing.
This test should not be ordered more than ONCE per episode of diarrhea (at least 1 week between collections).
Notifiable Conditions
The following are notifiable conditions under Wa State Law:
Campylobacter, Cryptosporidium, Shiga toxin producing E coli (including but not limited to ECO157), Cyclospora, Giardia, Salmonella, Shigella, Vibrio, and Yersinia.
See Link below for Notifiable Conditions for WA state labs:
https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions
See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:
https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf