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Test Code LAB252 Genital Wet Mount


Clinical System Name

Wet Mount


Trichomonas Smear

Wet Mount for Trichomonas vaginalis

Vaginal Wet Mount



Clue Cells


Sample Requirements

Specimen: Vaginal, Urethral or Penile Discharge, Urethral-mucosa scraping, collected with a BD ESwab.

Container: Swab is placed in the tube containing Amies transport media that comes packaged with the swab.

Preferred Vol: N/A

Minimum Vol: N/A


Specimen must be examined within 1 hour of collection to detect motile Trichomonas and sperm.

If it is not possible to examine specimen within 1 hour, it is suggested to order Trichomonas vaginalis amplified nucleic acid detection to be tested at the University of Washington laboratory. This test requires an additional swab collection using the APTIMA swab kit. 

Processing Instructions

CPA: Send sample to Microbiology immediately for processing.  


Off-site collection: Seattle Children's South Clinic laboratory: set up and read the genital wet mount on-site in order to observe viable Trichomonas. Sample should be retained at room temperature and sent to the SCH Microbiology laboratory for confirmatory examination.

All other off-site Children's Lab locations should send the sample to the main hospital laboratory immediately (direct). Keep sample at room temperature. Providers are encouraged to order Trichomonas vaginalis amplified nucleic acid detection at UW if confirmatory testing is desired (requires second swab in APTIMA collection kit).


Temp Time
Room temp 24 hours (initial exam within 1 hour of collection)
Refrigerated N
Frozen N



Procedure Performed Turn Around Time (TAT)
Genital Wet Mount 24/7

1 hour


Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes




Genital wet mounts are examined for the following significant clinical findings: Trichomonas vaginalis, Polymorphonuclear Leukocytes (Neutrophils), Sperm, Yeast, and Clue Cells. The presence of any of these forms is considered a positive result. 

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reporting Information

Preliminary Report: 1 hour

Final Report: 24 hours