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HelpTest Code LAB252 Genital Wet Mount
Infectious
Clinical System Name
Wet Mount
Synonyms
Trichomonas Smear
Wet Mount for Trichomonas vaginalis
Vaginal Wet Mount
Sperm
Yeast
Clue Cells
LAB252
Sample Requirements
Specimen: Vaginal, Urethral or Penile Discharge, Urethral-mucosa scraping, collected with a BD ESwab.
Urine is acceptable, but is not considered an optimal specimen for this test. A urinanlysis with microscopic exam is preferred.
Container: Swab is placed in the tube containing Amies transport media that comes packaged with the swab.
Urine in a sterile urine collection cup.
Preferred Vol: N/A
Minimum Vol: N/A
Specimen must be examined within 1 hour of collection to detect motile Trichomonas and sperm.
If it is not possible to examine specimen within 1 hour, it is suggested to order Trichomonas vaginalis amplified nucleic acid detection to be tested at the University of Washington laboratory. This test requires an additional swab collection using the APTIMA swab kit. It should be ordered in Epic as a MISC test and transported room temperature. Refer to the link above for instructions from UW.
Processing Instructions
CPA: Send sample to Microbiology immediately for processing.
Off-site collection: Seattle Children's South Clinic laboratory: set up and read the genital wet mount on-site in order to observe viable Trichomonas. Sample should be retained at room temperature and sent to the SCH Microbiology laboratory for confirmatory examination.
All other off-site Children's Lab locations should send the sample to the main hospital laboratory immediately (direct). Keep sample at room temperature. Providers are encouraged to order Trichomonas vaginalis amplified nucleic acid detection at UW if confirmatory testing is desired (requires second swab in APTIMA collection kit).
Stability
| Temp | Time |
|---|---|
| Room temp | 24 hours (initial exam within 1 hour of collection) |
| Refrigerated | N |
| Frozen | N |
Availability
| Procedure | Performed | Turn Around Time (TAT) |
|---|---|---|
| Genital Wet Mount | 24/7 |
1 hour |
Performing Laboratory
Seattle Children's Laboratory
Department
Microbiology Laboratory
CPT Codes
87210
Description
Genital wet mounts are examined for the following significant clinical findings: Trichomonas vaginalis, Polymorphonuclear Leukocytes (Neutrophils), Sperm, Yeast, and Clue Cells. The presence of any of these forms is considered a positive result.
Notifiable Result
See Link below for Notifiable Conditions for WA state labs:
https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions
See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:
https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf
Reporting Information
Preliminary Report: 1 hour
Final Report: 24 hours