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Test Code LAB2543 Culture, Resistant Enterics Surveillance

Infectious

Clinical System Name

Culture, Resistant Enterics Surveillance

Synonyms

Enteric Resistance Surveillance Screen

AmpC producer

Carbapenemase producer

Carbepenem Resistant Enterics

ESBL

Extended Spectrum Beta-lactamase Producer

Metalobetalactamase Producer

Multi Drug Resistant Enterics

MDR

Vancomycin Resistant Enterococcus

VRE

Stool Culture Resistance Screen

Stool MDR Screen 

LAB2543

Sample Requirements

Specimen: Stool or Lower Intestinal Tract Contents (preferred). Rectal swabs, rectal e-swabs, and samples in diapers are accepted, but are discouraged because of decreased test sensitivity.

Container: Sterile Container

Preferred Vol: 0.5 grams or mL

Minimum Vol: 0.2 grams or mL

 

 

Processing Instructions

CPA: Send sample to Microbiology for processing. If sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.

 

Off-site collection:  Off-site Children's Lab locations should send to SCH Main Campus for testing. Refrigerate sample before and during courier transport.

 

Other off-site locations, refrigerate sample prior to and during transport to the SCH Microbiology Laboratory. 

Stability

Temp Time
Room temp 2 hours
Refrigerated 8 hours
Frozen N

Availability

Procedure Performed Turn Around Time (TAT)
Culture 24/7 48 - 72 hour

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87081

 

This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Description

Resistant Enteric Surveillance Screen is intended as a screen for colonization of anitbiotic resistant enteric organisms (ex: VRE, ESBL, CRE, etc) which have important Infection Control implications. It is primarily used in the Hem/Onc and SCCA populations to determine if a patient is colonized with these organisms. This culture is not suitable for ruling out enteric pathogens (see Stool Culture).

 

Stool samples are preferred for this test, as the organisms will persist in the stool even after other sites have cleared. Specimen collection by rectal swabs, rectal e-swabs, or stool collected in diapers may significantly compromise test sensitivity. These other specimens will be accepted, but are strongly discouraged.

 

After antibiotic therapy is completed for VRE or an MDR-GNR, a patient should have 3 consecutive negative cultures collected at least 1 week apart to ensure clearance of the organism. If one or more of the surveillance cultures is positive, repeat surveillance testing should not be done until at least 90 days have passed.  See Children's Policy Resistant Organisms: Management of Patients with MRSA, VRE, CRE, or other Multi-Drug-Resistant Gram Negative Bacilli on CHILD for additional information about Resistant Organisms sample collection.

Reporting Information

Negative Final Report: 48 hours
 

Positive Final Report: 48-96 hours. A finding of Vancomycin Resistant Enterococcus (VRE), or Multiple Drug Resistant Gram Negative Rod (MDRGNR) is considered a positive result. This information will be called to the provider if the patient has not had the organism previously. Infectious Disease Consult is recommended for treatment options.

Notifiable Result

Carbapenem-Resistant Enterobacteriaceae is considered a Notifiable Condition by Wash State law. See link below for definition and reporting information.

 

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf