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HelpTest Code LAB2581 Sputum Culture
Infectious
Clinical System Name
Sputum Culture
Sample Requirements
Specimen: Sputum (expectorated) from a deep cough.
Container: Sterile Container
Preferred Vol: N/A
Minimum Vol: N/A
*Samples which are predominantly saliva contain epithelial cells and a mixture of micoorganisms from the oropharynx. Such samples will not yield clinically relevant information. Samples with >10 epithelial cells per low power field on microscopic examination will be rejected. The provider will be contacted for repeat collection.
For Cystic Fibrosis patients only, see Culture, CF Respiratory (SPUTUM,BAL) QUANTitative.
Processing Instructions
CPA: Send to Microbiology for processing.
Off-site collection: Sputum cultures are not set up at Off-site Children's Lab locations. Refrigerate sample until and during transport to SCH Micro.
Other off-site locations, refrigerate sample until and during transport to SCH Micro. Sample must be received into the microbiology laboratory within 24 hours of collection.
Stability
| Temp | Time |
|---|---|
| Room temp | 2 hours |
| Refrigerated | 24 hours |
| Frozen | N |
Availability
| Procedure | Performed | Turn Around Time (TAT) |
|---|---|---|
| Gram Stain | 24/7 | 2 hours |
| Culture | 24/7 | 48-72 hours |
Performing Laboratory
Seattle Children's Laboratory
Department
Microbiology Laboratory
CPT Codes
87070
87205 (Gram Stain)
This test is not limited to the CPT codes listed. Others may be added as more testing is necessary and depending on the pathogens isolated (example: MIC's and ID codes)
Description
Includes Bacterial Culture and Gram Stain. Specimen cultured for Aerobic bacteria.
Notifiable Result
See Link below for Notifiable Conditions for WA state labs:
https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions
See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:
https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf
Reporting Information
Preliminary Report: 24 hours
Negative Final Report: 48 hours