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Test Code LAB2585 Wound, Hardware Associated Culture


Clinical System Name

Wound, Hardware Associated Culture


Deep Wound, Hardware associated

Anaerobic Culture



Hardware Bone

Hardware Abscess

Hardware Fluid

Hardware Tissue


Sample Requirements

Specimen: BD ESwab, Tissue sample, or piece of hardware.

Container: ESwab should be inserted into the tube of Aimes transport media that is provided in the swab collection kit. Tissue or hardware should be placed into sterile container.

Preferred Vol: N/A

Minimum Vol: N/A


Please consult the Photo Swab Guide to determine which swab to collect.


Main Campus collection: Sample should be collected on a BD ESwab, in a sterile syringe, or sterile cup  (for larger volumes) . Note that the BD ESwab (available from Central Service and Demand Flow carts) are acceptable for both culture and Gram stain.


Regional Clinic Collection: Samples collected at Regional Clinic locations (where specimen set up will be delayed) are optimally collected on a BD ESwab Amies transport media. Samples collected on ESwabs swabs can also be used for Gram Stain and are stable at room temperature for up to 24 hours for transport to SCH.
Sterile fluid samples can be collected in a syringe or sterile cup.
Tissue samples or Hardware samples should be collected into a sterile container.


Processing Instructions

CPA: Send sample to Microbiology for processing.  If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.


Regional Clinic collection: Samples collected on BD ESwabs are sent to Main Campus Microbiology for culture set up. Swabs should be retained at room temperature until and during courier transport.


Fluid sent in a capped syringe should be set up at the Off-site Labs per Lucidoc instructions. After set up, refrigerate remaining sample. Incubate plates until courier transport.


Tissue sent in sterile container should be sent via cab to SCH Micro for immediate culture set up. Overlay large tissue pieces with Thio broth to keep tissue moist.

Note: if tissue piece is small it can be placed in an ESwab Transport media tube. This tube can then be kept at room temperature until courier pick up.  





BD ESwab or small piece of tissue
Temp Time
Room temp 24 hours  
Refrigerated OK for up to 48 hours
Frozen N



Tissue sample in sterile container with Thio broth
Temp Time
Room temp 1 hour
Refrigerated N
Frozen N



Procedure Performed Turn Around Time (TAT)
Gram Stain 24/7

1 hour

Culture 24/7 12 days

Cultures are read at appropriate intervals to detect early bacterial growth. STAT requests are not applicable.

Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes



87205 (Gram Stain)

This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)


Includes Bacterial Culture and Gram Stain. Specimen is cultured for Aerobic and Anaerobic bacteria. This culture type is held for 10 days to allow cultivation of fastidious and/or slow growing organisms that are often associated with hardware infections (ex: Cutibacterium).

Reporting Information

Preliminary Report:  18 - 24 hours

Negative Final Report: 12 days


Broth culture incubation is automatically extended up to 10 days for Hardware Associated Wound Cultures.

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State: