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Test Code LAB266 Acid Fast Stain


Clinical System Name

AFB Stain


AFB Stain

AFB Smear

Acid Fast Stain

Acid Fast Bacilli

Auramine Stain

Modified Kinyoun


Atypical Bacteria


Sample Requirements

Specimen: Culture Specimen

Container: Sterile Container

Preferred Vol: N/A

Minimum Vol: N/A

Processing Instructions


CPA: Send sample to Microbiology for processing.  If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.


Off-site collection: Off-site Children's Lab locations should send the specimen to the SCH Microbiology Laboratory for processing. Sample should be refrigerated prior to and during courier transport. 


Other off-site locations, refrigerate sample prior to and during courier transport to SCH Microbiology Laboratory.


Temp Time
Room temp 30 min

24 hours

Respiratory samples up to 5 days.

Frozen N



Procedure Performed Turn Around Time (TAT)
AFB Stain 24/7

24 hours


Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes



This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (example: MIC's and ID codes)


Includes Stain for Acid Fast Bacilli only. Please note that AFB Culture order includes an AFB Stain. An AFB culture is recommended and more sensitive than an AFB stain alone.

Note AFB stains are not performed on Blood samples, see AFB Blood Culture.

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reporting Information

Final Report: 24 hours