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HelpTest Code LAB2686 1,25-Dihydroxyvitamin D, Serum
Reporting Name
1,25-Dihydroxyvitamin D, SSCH Clinical System Name: 1,25-Dihydroxy Vitamin D Level
SCH Procedure Name: Calcitriol (1,25-Dihydroxy Vitamin D) Level
Useful For
Evaluation of selected patients with disorders of calcium and phosphate metabolism
Evaluation of chronic kidney disease with suspected abnormalities of vitamin D metabolism
Investigation of suspected hereditary disorders of vitamin D metabolism, including vitamin D-dependent rickets
Evaluation of parathyroid hormone-independent hypercalcemia, particularly when granulomatous disease, lymphoma, or CYP24A1 deficiency is suspected
As a second-line test following measurement of 25-hydroxyvitamin D when clinically indicated
Synonyms:
Renal Failure
Bone Disease
Calcitriol
Ordering Guidance
The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) may be needed to adequately assess vitamin D status.
Specimen Required
Patient Preparation:
Fasting: 4 hours, preferred but not required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Collect AT LEAST 3 mL WHOLE BLOOD in a red or gold top tube.
Specimen Type
SerumSpecimen Minimum Volume
Serum: 0.7 mL
Serum
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 30 days |
| Frozen | 30 days | |
| Ambient | 7 days |
Day(s) Performed
Monday through Friday
TAT: 4 - 6 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
Deuterated isotopically labeled 1,25-dihydroxyvitamin D2 and D3 are added to the sample as internal standards. 1,25-Dihydroxyvitamin D2 and D3 and the internal standards are extracted using immunopurification. Extracts are then derivatized and analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Reference Values
Males:
<16 years: 24-86 pg/mL
≥16 years: 18-64 pg/mL
Females:
<16 years: 24-86 pg/mL
≥16 years: 18-78 pg/mL
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82652
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DHVD | 1,25-Dihydroxyvitamin D, S | 62290-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8822 | 1,25-Dihydroxyvitamin D, S | 62290-2 |
Report Available
2 to 5 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Renal Diagnostics Test Request (T830)
Aliases: 1,25-Dihydroxy Vitamin D, 1,25-Dihydroxycholecalciferol, 1,25-Dihydroxyvitmin D3, Calcitriol