Sign in →

Test Code LAB2686 1,25-Dihydroxyvitamin D, Serum

Important Note

This test is privliged to Seattle Children's Endocrinologists and Nephrologists, and can be ordered directly from select Epic powerplans. All other providers should order a Miscellaneous Test.

Reporting Name

1,25-Dihydroxyvitamin D, S
Seattle Children's Hospital Note:

SCH Clinical System Name: 1,25-Dihydroxy Vitamin D Level

SCH Procedure Name: Calcitriol (1,25-Dihydroxy Vitamin D) Level

Useful For

As a second-order test in the assessment of vitamin D status, especially in patients with renal disease

 

Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxyvitamin D)

 

Differential diagnosis of hypercalcemia

Seattle Children's Hospital Note:

Synonyms:

Renal Failure

Bone Disease

Calcitriol


Ordering Guidance


The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) may be needed to adequately assess vitamin D status.



Specimen Required


Patient Preparation: Fasting is preferred for 4 hours but not required.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect  AT LEAST 3 mL WHOLE BLOOD in a red or gold top tube.

Specimen Type

Serum

Specimen Minimum Volume

0.7 mL

Seattle Children's Hospital Note:

Serum

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
  Ambient  7 days

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 4 - 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Method Description

Deuterated stable isotopes are added to sample as internal standard. 1,25-Dihydroxyvitamin D and the internal standard are extracted. The extracts are then further purified by solid phase extraction (SPE) and affinity extraction. Extracts are then derivatized and analyzed by chromatography-tandem mass spectrometry using multiple reaction monitoring.(Unpublished Mayo method)

Reference Values

Males:

<16 years: 24-86 pg/mL

≥16 years: 18-64 pg/mL

 

Females:

<16 years: 24-86 pg/mL

≥16 years: 18-78 pg/mL

 

For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82652

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHVD 1,25-Dihydroxyvitamin D, S 62290-2

 

Result ID Test Result Name Result LOINC Value
8822 1,25-Dihydroxyvitamin D, S 62290-2

Report Available

2 to 5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Renal Diagnostics Test Request (T830)

Seattle Children's Hospital Additional Information:

Aliases: 1,25-Dihydroxy Vitamin D, 1,25-Dihydroxycholecalciferol, 1,25-Dihydroxyvitmin D3, Calcitriol