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Test Code LAB2770 Alpha-1-Antitrypsin Phenotype, Serum

Reporting Name

Alpha-1-Antitrypsin Phenotype
Seattle Children's Hospital Note:

SCH Clinical System Name: Alpha 1 Antitrypsin Phenotype

Useful For

Identification of homozygous and heterozygous phenotypes of the alpha-1-antitrypsin deficiency


Specimen Required


Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1.25 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Seattle Children's Hospital Note:

Collect 1.25 mL whole blood in a Red top.

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Day(s) Performed

Monday through Friday

Seattle Children's Hospital Note:

TAT: 4 - 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

A1AP2: Isoelectric Focusing

AATP: Nephelometry

Method Description

Phenotyping is done by isoelectric focusing in agarose gels.(Package insert: Hydragel 18 A1AT Isofocusing. Sebia. 2013 July)

 

Nephelometry.(Instruction manual: Siemens Nephelometer II. Siemens, Inc., Newark, DE)

Reference Values

ALPHA-1-ANTITRYPSIN

100-190 mg/dL

 

ALPHA-1-ANTITRYPSIN PHENOTYPE

The interpretive report will identify the alleles present. For rare alleles, the report will indicate whether or not they have been associated with reduced quantitative levels of alpha-1-antitrypsin.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82103

82104

 

LOINC Code Information

Test ID Test Order Name Order LOINC Value
A1APP Alpha-1-Antitrypsin Phenotype 32769-2

 

Result ID Test Result Name Result LOINC Value
AATP Alpha-1-Antitrypsin, S 6771-0
8166 Alpha-1-Antitrypsin Phenotype 32769-2

Profile Information

Test ID Reporting Name Available Separately Always Performed
A1AP2 Alpha-1-Antitrypsin Phenotype No Yes
AATP Alpha-1-Antitrypsin, S Yes, (Order AAT) Yes

Testing Algorithm

See Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm in Special Instructions.

Report Available

2 to 6 days

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Forms

If not ordering electronically, complete, print, and send 1 of the following with the specimen:

-Gastroenterology and Hepatology Test Request (T728)

-General Request (T239)

Seattle Children's Hospital Additional Information:

Aliases: A1A Phenotyping; a-1-Antitrypsin, Phenotype; AAT Phenotyping; Alpha 1 Antitrypsin; Anti-Alpha-1-Trypsin; Antitrypsin; Antitrypsin, Phenotype; Pi Typing (Alpha-1-Antitrypsin Phenotyping); Protease Inhibitor Allo Typing; Alpha-1-Antitrypsin Reflex