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HelpTest Code LAB2802 Proteinase 3 Antibodies
Clinical System Name
Proteinase 3 Antibodies
Synonyms
ANCA C
ANCA-C
Anti-Proteinase 3 (C-ANCA)
C ANCA
C-ANCA
ANTI PR3
Sample Requirements
Specimen: Whole Blood
Container(s): Gold SST; Red also acceptable
Preferred Vol: 2 mL
Minimum Vol: 1 mL
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2-8C
Storage location: CPA refrigerator, FrigF rack.
Off-site collection: Avoid hemolysis. Spin blood, refrigerate 1 mL serum in a polypropylene aliquot tube.
Stability
| Temperature | Time |
|---|---|
| Room temp | |
| Refrigerated | 7 d |
| Frozen | 6 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N* | M-F | 1-3 d |
*Available STAT only upon approval of Seattle Children's Hospital Pathologist on call.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
| CPT CODE(S) |
|---|
| 86021 |
Methodology
Method: Immunoassay on Bioplex 2200
Analytical Volume: 0.4 mL serum
Limitations:
Reference Range
| AI | |
|---|---|
| Negative | < 0.4 |
| Equivocal | 0.4 - 0.9 |
| Positive | > 1.0 |
Description
This assay is intended for the detection of IgG class antibodies to PR3 (Proteinase 3) in human serum. It is used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of anti-neutrophil cytoplasmic antibodies (ANCA), especially microscopic polyangitis (MPA). May also be useful to monitor treatment response in MPA. The reported result is semi-quantitative.