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HelpTest Code LAB2806 Antigen Stimulation Study
Clinical System Name
Antigen Stimulation Study Request
Synonyms
Lymphocyte Antigen Stimulation
Lymphocyte Function Analysis
T cell proliferation to antigens
Antigen Stimulation Study
AG STIM
Sample Requirements
Specimen: Whole Blood
Container(s): Dark Green/Sodium Heparin (no serum separator)
Preferred Vol: 10 mL
Minimum Vol: 10 mL (if unable to collect 10 mL, please contact lab at 206-987-2560; see note above)
Note:
Specimens stable for only 24 hours at room temperature.
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH South and SCH North) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | must be received before 12 noon Friday | must be received at SCH main lab before 12 noon Friday |
| Saturday | do not collect | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon day before holiday |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Call the Cell Marker Lab (206) 987-2560 with any questions regarding specimen collection or handling. If unable to collect minimum volume, please collect as close to 10 mL as possible (10 mL each for Mitogen and Antigen) and notify Cell Markers at 206-987-2560 (leave voicemail after hours).
Draw an additional 10.0 mL if a mitogen (PHA, CD3) is also ordered.
Processing Instructions
Reject due to: n/a - send to lab
Spin: N
Aliquot: N
Temp: RT
Storage location: Days: Transport specimen, (copy of community services requisition, if applicable), and labels to the Cell Markers Lab (station #181). Notify Cell Markers at x72560 right away if specimen is near 24 hour specimen stability limit - billing/ordering problems should not delay delivery to lab. Eves/Nights: Store specimen, (copy of community services requisition, if applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: Dark Blue Na Heparin ok. A normal control should accompany the specimen. It is critical that samples be kept at room temperature; use extra packing to maintain temperature. If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning. Testing must be set up within 24 hours of collection. Draw an additional 10 mL if an mitogen (PHA, CD3) is also ordered. Transport all tubes at RT to Seattle Children's Hospital address on requisition: Laboratory FB.2.441, 4800 Sand Point Way NE, Seattle, WA 98105.
Stability
| Temperature | Time |
|---|---|
| Room temp | 24 hours |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
Monday - Thurs; Friday must be in lab before noon |
7 - 10 days |
Limited specimen stability - see specimen requirements.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| CPT CODE | |
|---|---|
| Set up and Initial Mitogen or Antigen Stimulant |
86353 |
| Each additional Mitogen or Antigen Stimulant | 86353 |
Methodology
Method: Lymphocyte proliferation with 3H thymidine incorporation
Analytical Volume: dependent on absolute lymphocyte count
Limitations:
Reference Range
Reference values accompany patient report.
Description
The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).