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Test Code LAB2827 B Cell Phenotyping

Important Note

Note: limited specimen stability - see Sample Requirements below.  DO NOT REFRIGERATE

Do not collect on a Friday before a holiday Monday.

Offsite collection: See additional notes under 'Processing Instructions - Offsite Collection'

Please note this is not a test for Lymphocyte Subsets, please see our T&B Testing under Lymphocyte Subsets.


Clinical System Name

B Cell Phenotyping


B Cell Immunophenotyping

Switched/Unswitched Memory B cells

B Cell Developmental Subsets

B Cell Phen

Sample Requirements

Specimen:  Whole Blood

Container(s):  Dark Green/Sodium Heparin (no serum separator)

Preferred Vol:  5.0 mL

Minimum Vol:  5.0 mL (if unable to collect 5.0 mL, please contact lab at 206-987-2560)



  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue, SCH North, and SCH South)
Saturday-Thursday collect any time collect any time
Friday Collect any time, except do not collect on a Friday before a holiday Monday. Collect any time, except do not collect on a Friday before a holiday Monday.


Call the Cell Marker Lab (206) 987-2560 with any questions regarding specimen collection or handling.


Draw an additional 3.0 mL (1.0 mL minimum) and see separate listing for T Cell Phenotyping if this test is also ordered (different shipping instructions/performed at Mayo Medical Laboratories).



Processing Instructions

Reject due to: Clotted specimens, microtainers, refrigerated, samples received > 96 hours post-collection

Spin: N

Aliquot: N

Temp: Room Temperature

Storage location: Days:  Transport specimen, copy of community services requisition (if applicable), and labels to 10th floor Cell Markers (station #280).  Eves/nights: Store specimen, copy of community services requistion, if applicable, and labels in the Cell Markers RT box in CPA.


Off-site collection: It is critical that the samples be kept at room temperature; use extra packing to maintain temperature. We recommend collecting specimens as late in the day as possible, and sending via overnight delivery to allow testing to be set up the following morning. Transport specimen (for B cell phenotyping) at RT to Seattle Children's Hospital address on requisition: Laboratory OC.8.740, 4800 Sandpoint Way NE, Seattle, WA  98145.




Specimen Type Temperature Time
Whole Blood Room temp 96 h
  Refrigerated N
  Frozen N



STAT Performed TAT
N Monday - Friday 3-4 weeks


Performing Laboratory

Seattle Children's Laboratory


Department:  Cell Markers

Phone:  206-987-2560


Lab Client Services: 206-987-2617,

CPT Codes

88184, 88185 (x11), 88188


Method: Peripheral blood mononuclear cells are stained with fluorescently-labelled antibodies to:  CD10, CD19, CD20, CD21, CD24, CD27, CD38, IgA, IgD, IgG, IgM, and BAFF-R. Cells are analyzed by flow cytometry and specific cell populations are identified by cell-surface marker phenotype.

Reference Range

Interpretive report will be provided


see Cell Markers requisition

Clinical Utility

Clinical Utility