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HelpTest Code LAB2827 B Cell Phenotyping
Clinical System Name
B Cell Phenotyping
Synonyms
B Cell Immunophenotyping
Switched/Unswitched Memory B cells
B Cell Developmental Subsets
B Cell Phen
Sample Requirements
Specimen: Whole Blood
Container(s): Sodium Heparin- no serum separator (dark green or dark blue)
Preferred Vol: 5.0 mL
Minimum Vol: 5.0 mL (if unable to collect 5.0 mL, please contact lab at 206-987-2560)
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North, and SCH South) | |
|---|---|---|
| Saturday-Thursday | collect any time | collect any time |
| Friday | Collect any time, except do not collect on a Friday before a holiday Monday. | Collect any time, except do not collect on a Friday before a holiday Monday. |
Call the Cell Marker Lab (206) 987-2560 with any questions regarding specimen collection or handling.
Draw an additional 3.0 mL (1.0 mL minimum) and see separate listing for T Cell Phenotyping if this test is also ordered (different shipping instructions/performed at Mayo Medical Laboratories).
CallCalC
Processing Instructions
Reject due to: Clotted specimens, microtainers, refrigerated, samples received > 96 hours post-collection
Spin: N
Aliquot: N
Temp: Room Temperature
Storage location: Days: Transport specimen, copy of community services requisition (if applicable), and labels to the Cell Markers Lab (station #181). Eves/nights: Store specimen, copy of community services requistion, if applicable, and labels in the Cell Markers RT box in CPA.
Off-site collection: It is critical that the samples be kept at room temperature; use extra packing to maintain temperature. We recommend collecting specimens as late in the day as possible, and sending via overnight delivery to allow testing to be set up the following morning. Transport specimen (for B cell phenotyping) at RT to Seattle Children's Hospital address on requisition: Laboratory OC.8.740, 4800 Sandpoint Way NE, Seattle, WA 98145.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood-Na Hep | Room temp | 96 h |
| Refrigerated | N | |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Monday - Friday | 3-4 weeks |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone: 206-987-2560
Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org
CPT Codes
88184, 88185 (x11), 88188
Methodology
Method: Peripheral blood mononuclear cells are stained with fluorescently-labelled antibodies to: CD10, CD19, CD20, CD21, CD24, CD27, CD38, IgA, IgD, IgG, IgM, and BAFF-R. Cells are analyzed by flow cytometry and specific cell populations are identified by cell-surface marker phenotype.
Reference Range
Interpretive report will be provided
Requisition
Clinical Utility
Clinical Utility