Home
HelpTest Code LAB2834 BCR/ABL p190, Quant
Additional Codes
BCR/ABL p190
Description
The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR.
Clinical System Name
BCR/ABL p190, Quant
Synonyms
B-ALL; BALL; B-Cell Acute Lymphobastic Leukemia; P190; Philadelphia chromosome; QP190; Quantitative BCR/ABL p190; t(9;22); translocation (9;22)
Sample Requirements
Specimen: Blood or Bone Marrow
Container(s): Lavender Top Tube
Preferred Vol: 6.0 mL Blood or 2.0 mL Bone Marrow
Minimum Vol: 4.0 mL Blood or 1.0 mL Bone Marrow
Processing Instructions
Reject due to: Specimens with low volume or inadequate WBC. Specimens received beyond stability limit. Tissue, Decalcified or unacceptable fixatives.
Spin: N
Aliquot: N
Temp: 2 - 4 C
Storage Location: Store sample in CPA refrigerator Send Outs rack. On weekends, send sample refrigerated to UW NW220 ASAP so that testing can be run first thing Monday morning.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Room Temp | <24 h | |
| Refrigerated | 4 d | |
| Frozen | Unacceptable |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Tu, Th | 5 d |
Performing Laboratory
University of Washington Medical Center
Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109
Phone Number: (206) 606-7060
Hours:
- 7:00 am - 5:30 pm M-F
- 8:00 am - 4:30 pm Saturday
- Closed on Sundays, reduced staffing on major holidays
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: LDT, RT-PCR
Analytical Volume: 4.0 mL Blood or 1.0 mL Bone Marrow
Limitations: This assay in unable to detect BCR::ABL1 p190, p230, or other rare transcripts.
Reference Range
See individual components.
Send Out Instructions
| Reference Lab Test Name: | BCR/ABL p190, Quant by RT PCR |
| Reference Lab Test Number: | QP190 |
| Instructions: | Send out daily with the UW courier. |