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HelpTest Code LAB2835 BCR/ABL p210, Quant
Additional Codes
BCR/ABL p210
Description
This assay is a nucleic acid amplification test for the quantitation of BCR::ABL1 p210 transcripts (e13a2 and e14a2) in total RNA from whole blood and bone marrow. This quantitative test is recommended for therapeutic monitoring and detection of minimal residual disease for patients with an established diagnosis. The test does not differentiate between e13a2/b2a2 or e14a2/b3a2 fusion transcripts and does not detect other rare fusion transcripts resulting from t(9;22).
Clinical System Name
BCR/ABL p210, Quant
Synonyms
B-ALL; BALL; B-Cell Acute Lymphoblastic Leukemia; BCR-abl detection at FHCRC; Chronic Myelogenous Leukemia; CML; P210; Philadelphia chromosome; QP210; Quantitative BCR/ABL p210; t(9;22); translocation (9;22)
Sample Requirements
Specimen: Blood or Bone Marrow
Container(s): Lavender Top Tube
Preferred Vol: 6.0 mL Blood or 2.0 mL Bone Marrow
Minimum Vol: 4.0 mL Blood or 1.0 mL Bone Marrow
Processing Instructions
Reject due to: Serum, plasma, extracted DNA, CSF, FFPE tissue, frozen whole blood or bone marrow. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens. Specimens received beyond 72-hr stability limit.
Spin: N
Aliquot: N
Temp: 2 - 4 C
Storage Location: Store sample in CPA refrigerator Send Outs rack. On weekends, send sample refrigerated to UW NW220 ASAP so that testing can be run first thing Monday morning.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Room Temp | <24 h | |
| Refrigerated | 72 h | |
| Frozen | Unacceptable |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M - F | 4 d |
Performing Laboratory
University of Washington Medical Center
Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109
Phone Number: (206) 606-7060
Hours:
- 7:00 am - 5:30 pm M-F
- 8:00 am - 4:30 pm Saturday
- Closed on Sundays, reduced staffing on major holidays
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: RT-PCR
Analytical Volume: 4.0 mL Blood or 1.0 mL Bone Marrow
Limitations: This assay in unable to detect BCR::ABL1 p190, p230, or other rare transcripts.
Reference Range
See individual components.
Send Out Instructions
| Reference Lab Test Name: | BCR/ABL p210, Quant by RT PCR |
| Reference Lab Test Number: | QP210 |
| Instructions: | Send out daily with the UW courier. |